LOCUS       sequence-134172-         642 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Cloned region contains Acetobacter aceti 1023 purE(H59N) gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK340
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             571..579
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(595..612)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(595..611)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(604..626)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aaaaaccgga tagataccca ccgtgagcga aaccgccccc ctgccctctg cttccagtgc
       61 tttagaagat aaagcagcct ctgcccccgt tgtgggcatt atcatgggca gccagtcaga
      121 ctgggaaacc atgcgccatg cagacgcatt gctgacagag ctggaaattc cgcatgaaac
      181 cctcattgtt tcagcaaatc gtacgccaga ccggttggcg gattatgcgc gtacggcagc
      241 cgaacgcggc ctgaatgtga ttattgctgg ggccggtgga gctgcacatc tgccgggtat
      301 gtgtgcggca tggacacgcc tgcccgtgct gggtgttcca gtagaatctc gcgctcttaa
      361 aggcatggac agcctgcttt ccattgtgca gatgcccggc ggcgtgcctg tgggcacttt
      421 ggctattggg gcatccggcg ccaaaaatgc cgccttgctg gccgcctcca ttcttgcact
      481 gtataacccg gcccttgccg cacgccttga aacatggcgc gcattgcaga cggcctctgt
      541 ccccaactct cccattaccg aagataaatg acctccttac attctaagct tggcactggc
      601 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt ac
//
