LOCUS       sequence-134241-        1081 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, Lys41>Met/Phe79>Trp mutant (TdPurE2-K41M,F79W).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Kappock
  TITLE     Serine mutants of Treponema denticola PurE2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK660
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             599..621
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(652..671)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(653..671)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcga gctccgtcga caagaaactg aaacgctgaa ttcagcgttt
      121 cagtttcgaa tcgtcttcga taattttctg cgcattggat tccatatagg atttgaccga
      181 atctgcgatt tctttatcgt acagtgaaaa aatacgagcc gccaggagtg ccgcattttt
      241 cggttcaaga accagtgccg gactaatccc gctcggcatc cgtaacgaag aataaatgtc
      301 ggcgccggcg aagctatcgc ttggcggtgg gcaggcaatg gttgcacctt tgacccagcc
      361 atcaacaaag cctgataaag cgttactacg cccagcgatc gtgatgtaca gtttaggacg
      421 gtccaaagct tcatattctt tcagcataga cactacatgc tcagctgtca tgtgcgcgga
      481 gccgatgcga atggcatact caataccaaa agttttcaat tcagacgcaa ttttctcggc
      541 gtgacccata tctgaactgc tgcccataag gataatcacc agcggacgca tatgtatatc
      601 tccttcttaa agttaaacaa aattatttct agagggaaac cgttgtggtc tccctatagt
      661 gagtcgtatt aatttcgcgg gatcgagatc tcgggcagcg ttgggtcctg gccacgggtg
      721 cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt
      781 tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct
      841 gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg
      901 cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta
      961 ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt
     1021 ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg
     1081 g
//
