LOCUS       sequence-134791-        8792 bp    DNA     circular SYN 14-APR-2025
DEFINITION  This a retroviral expression plasmid expressing GFP tagged G12C 
            mutant Kras 2B oncogene along with hygro resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M,
            Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, 
            Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, 
            Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, 
            Psallidas I, Marazioti A, Stathopoulos GT
  TITLE     Mutant KRAS promotes malignant pleural effusion formation.
  JOURNAL   Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205.
  PUBMED    28508873
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-16"; volume: "8:15205. doi"; pages: " 
            10.1038/ncomms15205"
COMMENT     Sequence Label: 8798 GFP-KrasG12C_2B_hygro
FEATURES             Location/Qualifiers
     source          1..8792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(75..96)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(336..355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     683..704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1377..1929
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1521..1538)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1748..1767
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1963..3000
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(2998..3017)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3042..3090
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(3529..3548)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3530..3548)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3558..3574)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             3696..4210
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    4372..4388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4396..4402,4403..4420,4421..4426))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4441..4462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4579..4596)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4750..5338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4830..4849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5509..6300,6301..6369))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6132..6151
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6370..6474)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6542..6560
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6598..6620)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6720..6739
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6948..6964
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7163..7182
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             7382..7898
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    7962..8303
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             8370..8786
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     8716..8738
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     8754..8770
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 agatcgatcc agatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
       61 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      241 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      481 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
      541 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      601 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
      661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      721 ctctcggcat ggacgagctg tacaagtccg gactcagatc tatgactgag tataaacttg
      781 tggtggttgg agcttgtggc gtaggcaaga gcgccttgac gatacagcta attcagaatc
      841 actttgtgga tgagtacgac cctacgatag aggactccta caggaaacaa gtagtaattg
      901 atggagaaac ctgtctcttg gatattctcg acacagcagg tcaagaggag tacagtgcaa
      961 tgagggacca gtacatgaga actggggagg gctttctttg tgtatttgcc ataaataata
     1021 ctaaatcatt tgaagatatt caccattata gagaacaaat taaaagagta aaggactctg
     1081 aagatgtgcc tatggtcctg gtagggaata agtgtgattt gccttctaga acagtagaca
     1141 cgaaacaggc tcaggagtta gcaaggagtt acgggattcc gttcattgag acctcagcaa
     1201 agacaagaca gggtgttgac gatgccttct atacattagt ccgagaaatt cgaaaacata
     1261 aagaaaagat gagcaaagat gggaagaaga agaagaagaa gtcaaggaca aggtgtacag
     1321 ttatgtgaga attctgcagt cgacggtacc gcgggcccaa ccgccccccc cccctaacgt
     1381 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     1441 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     1501 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     1561 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     1621 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     1681 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     1741 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     1801 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     1861 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     1921 atgataatat ggccacaagt cgaggttggg gggcaatgag atatgaaaaa gcctgaactc
     1981 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     2041 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     2101 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     2161 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
     2221 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     2281 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     2341 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     2401 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     2461 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     2521 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     2581 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     2641 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     2701 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     2761 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     2821 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     2881 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     2941 gatagtggaa accgacgccc cagcactcgt ccggatcggg agatggggga ggctaactga
     3001 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     3061 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     3121 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     3181 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     3241 caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca tggggaatgg
     3301 tttatggttc gtgggggtta ttattttggg cgttgcgtgg ggtcaggtcc acgactggac
     3361 tgagcagaca gacccatggt ttttggatgg cctgggcatg gaccgcatgt actggcgcga
     3421 cacgaacacc gggcgtctgt ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg
     3481 atttctggcg tgccaagcta gtcgaggggg ggcccggtac ccaattcgcc ctatagtgag
     3541 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3601 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagagctta tcgataaaat
     3661 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     3721 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga
     3781 gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct
     3841 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc
     3901 cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     3961 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
     4021 tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg
     4081 atagactgcg tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
     4141 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
     4201 ggtctttcat gggtaacagt ttcttgaagt tggagaacaa cattctgagg gtaggagtcg
     4261 aatattaagt aatcctgact caattagcca ctgttttgaa tccacatact ccaatactcc
     4321 tgaaatagtt cattatggac agcgcaaaaa gagctgggga gaattgtgaa attgttatcc
     4381 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     4441 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     4501 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4561 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4621 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5161 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     5221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5401 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5521 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5581 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5641 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5701 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5761 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5821 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5881 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5941 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6001 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6061 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6121 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6181 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6241 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6301 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6361 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6421 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6481 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     6541 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     6601 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     6661 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     6721 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     6781 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     6841 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     6901 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     6961 cagtgccacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     7021 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     7081 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     7141 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     7201 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gcgattagtc caatttgtta
     7261 aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc
     7321 ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg
     7381 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
     7441 catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca
     7501 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     7561 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
     7621 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     7681 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     7741 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtattccca
     7801 ataaagcctc ttgctgtttg catccgaatc gtggactcgc tgatccttgg gagggtctcc
     7861 tcagattgat tgactgccca cctcgggggt ctttcatttg gaggttccac cgagatttgg
     7921 agacccctgc ccagggacca ccgacccccc cgccgggagg taagctggcc agcggtcgtt
     7981 tcgtgtctgt ctctgtcttt gtgcgtgttt gtgccggcat ctaatgtttg cgcctgcgtc
     8041 tgtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct
     8101 gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc
     8161 cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag
     8221 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc
     8281 gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga
     8341 ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga
     8401 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     8461 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     8521 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     8581 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     8641 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     8701 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     8761 cagccctcac tccttctcta ggcgccggaa tt
//
