LOCUS       sequence-135100-         842 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Encodes human full length SENP7. Suitable for in vitro 
            transcription/translation (T7)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Mendes AV, Grou CP, Azevedo JE, Pinto MP
  TITLE     Evaluation of the activity and substrate specificity of the human 
            SENP family of SUMO proteases.
  JOURNAL   Biochim Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020.
  PUBMED    26522917
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020."
COMMENT     Sequence Label: pET28a-HsSENP7
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatggtacct atggacaaga gaaagctcgg
      121 gcgacggcca tcttcatccg aaatcatcac agaaggaaaa aggaaaaagt catcttctga
      181 tttatcggag ataagaaaga tgttaaatgc aaaaccagag gatgtccatg ttcaatcacc
      241 actgtccaaa ttcagaagct cagaacgctg gactctccct ttgcagtggg aaagaagcct
      301 aaggaataaa gtcatctctc tagaccataa aaatcaaaaa catatccgag ggtgtcctgt
      361 tacttccaag tcatcaccag aaaggcaact caaagttatg ttgacgaatg tcctatggac
      421 ggatttagga cgaaaattca gaaagaccct acctagaaac gatgctaatt tatgtgatgc
      481 caacaaggtg caatcagact cattgccttc gacatctgtt gacagcctag agacatgtca
      541 aaaattagaa cctcttcgcc aaagccttaa tttatctgaa aggataccca gagttatatt
      601 gacgaatgtc ctgggaacgg agttaggaag aaaatacata aggaccccac ctgtaactga
      661 gggaagtttg agtgatacag acaacttgca atcagagcaa ctttcttcat catctgatgg
      721 cagcctagaa tcttatcaaa atctaaaccc tcacaagagc tgttatttat ctgaaagggg
      781 ctcacaacga agtaagacag tagatgacaa ttctgcaaag cagactgcgc acaataaaga
      841 aa
//
