LOCUS       sequence-135289-        9473 bp    DNA     circular SYN 15-APR-2025
DEFINITION  expresses 3*FLAG tagged human NSD3-short with 280-342 aa deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, 
            Pappin DJ, Joshua-Tor L, Vakoc CR
  TITLE     NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 
            Chromatin Remodeler.
  JOURNAL   Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033.
  PUBMED    26626481
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033."
COMMENT     Sequence Label: MSCV-NSD3-short del(280-342)
FEATURES             Location/Qualifiers
     source          1..9473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3171..3236
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        3253..3752
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3288..3311)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3685..3704
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3773..4372
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3773..3792)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4269..4289
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4433..5000
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4450..5002
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4594..4611)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4821..4840
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5014..5016,5017..5019,5020..5733)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5059..5080)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5320..5339)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5667..5688
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5835..6349
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6518..6534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6518..6534)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6531..6553)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6542..6558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6566..6572,6573..6590,6591..6596))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6611..6632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6749..6766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6920..7508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7000..7019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7679..8470,8471..8539))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8302..8321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8540..8644)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8712..8730
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8768..8790)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8890..8909
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9103..9125
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9253..9272
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9472..9473,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatccaccat ggatttctct ttctctttca
     1441 tgcaagggat catgggaaac acaattcagc aaccacctca actcattgac tccgccaaca
     1501 tccgtcagga ggatgccttt gataacaaca gtgacattgc tgaagatggt ggccagacac
     1561 catatgaagc tactttgcag caaggctttc agtacccagc tacaacagaa gatcttcctc
     1621 cactcacaaa tgggtatcca tcatcaatca gtgtgtatga aactcaaacc aaataccagt
     1681 catataatca gtatcctaat gggtcagcca atggctttgg tgcagttaga aactttagcc
     1741 ccactgacta ttatcattca gaaattccaa acacaagacc acatgaaatt ctggaaaaac
     1801 cttcccctcc acagccacca cctcctcctt cggtaccaca aactgtgatt ccaaagaaga
     1861 ctggctcacc tgaaattaaa ctaaaaataa ccaaaactat ccagaatggc agggaattgt
     1921 ttgagtcttc cctttgtgga gaccttttaa atgaagtaca ggcaagtgag cacacgaaat
     1981 caaagcatga aagcagaaaa gaaaagagga aaaaaagcaa caagcatgac tcatcaagat
     2041 ctgaagagcg caagtcacac aaaatcccca aattagaacc agaggaacaa aatagaccaa
     2101 atgagagggt tgacactgta tcagaaaaac caagggaaga accagtacta aaagaggaag
     2161 ccccagttca gccaatacta tcttctgttc caacaacgga agtgtccact ggtgttaagt
     2221 ttcaggttgg cgatcttgtg tggtccaagg tgggagcaac caaacaagcc agcaatcact
     2281 ctgagaaaca aaagattcgg aaaccccgac ctcagagaga acgtgctcag tgggatattg
     2341 gcattgccca tgcagagaaa gcattgaaaa tgactcgaga agaaagaata gaacagtata
     2401 cttttattta cattgataaa cagcctgaag aggctttatc ccaagcaaaa aagagtgttg
     2461 cctccaaaac cgaagttaaa aaaacccgac gaccaagatc tgtgctgaat actcagccag
     2521 aacagaccaa tgcaggggag gtggcctcct cactctcaag tactgaaatt cggagacata
     2581 gccagaggcg gcacacaagt gcggaagagg aagagccacc gcctgttaaa atagcctgga
     2641 aaactgcggc agcaaggaaa tccttaccag cttccattac gatgcacaaa gggagcctgg
     2701 atttgcagaa gtgtaacatg tctccagttg tgaaaattga acaagtgttt gctcttcaga
     2761 atgctacagg ggatgggaaa tttatcgatc aatttgttta ttcaacaaag ggaattggta
     2821 acaaaacaga aataagtgtc agggggcaag acaggcttat aatttctaca ccaaaccaga
     2881 gaaatgaaaa gccaacgcag agtgtatcat ctcctgaagc aacatctggt tctacaggct
     2941 cagtagaaaa gaagcaacag agaagatcaa ttagaactcg ttctgaatca gagaaatcca
     3001 ctgaggttgt gccaaagaag aagatcaaaa aggagcaggt tgaaacagtt cctcaggcta
     3061 cagtgaagac tggattacag aaagggtcgg cggaccgggg agtgcagggc tctgtcagat
     3121 tcagtgacag ctccgtctcc gcagcgattg aggaaactgt ggacggaggc gattacaaag
     3181 accatgacgg tgattataaa gatcatgata tcgattacaa ggatgacgat gacaagtgat
     3241 gagaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     3301 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     3361 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     3421 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     3481 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     3541 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     3601 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     3661 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     3721 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
     3781 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     3841 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     3901 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     3961 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     4021 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     4081 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     4141 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     4201 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     4261 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     4321 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     4381 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcca attccgcccc
     4441 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     4501 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     4561 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     4621 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     4681 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     4741 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     4801 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     4861 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     4921 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     4981 tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggcga ggagctgttc
     5041 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     5101 gtgtctggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     5161 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     5221 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     5281 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     5341 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5401 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5461 aacgtctata tcatggccga caagcagaag aacggcatca aggcgaactt caagatccgc
     5521 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     5581 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     5641 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     5701 atcactctcg gcatggacga gctgtacaag taatgaatta attaagaatt atcaagctta
     5761 tcgataccgt cgacctgcag ccaagcttat cgataaaata aaagatttta tttagtctcc
     5821 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     5881 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     5941 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     6001 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     6061 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     6121 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     6181 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
     6241 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     6301 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatg ggtaacagtt
     6361 tcttgaagtt ggagaacaac attctgaggg taggagtcga atattaagta atcctgactc
     6421 aattagccac tgttttgaat ccacatactc caatactcct gaaatagttc attatggaca
     6481 gcgcagaaga gctggggaga attaattcgt aatcatggtc atagctgttt cctgtgtgaa
     6541 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6601 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6661 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6721 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6781 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6841 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6901 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6961 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7021 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7081 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7141 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7201 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7261 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7321 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     7381 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7441 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7501 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7561 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7621 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7681 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7741 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7801 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7861 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7921 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7981 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8041 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8101 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8161 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8221 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8281 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8341 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8401 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8461 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8521 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8581 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8641 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     8701 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     8761 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     8821 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     8881 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     8941 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     9001 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     9061 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     9121 aaacgacggc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
     9181 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
     9241 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
     9301 gatgccggcc acgatgcgtc cggcgtagag gcgattagtc caatttgtta aagacaggat
     9361 atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta
     9421 cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg gaa
//
