LOCUS       sequence-135451-        1467 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Enomoto M, Bunge MB, Tsoulfas P
  TITLE     A multifunctional neurotrophin with reduced affinity to p75NTR 
            enhances transplanted Schwann cell survival and axon growth after 
            spinal cord injury.
  JOURNAL   Exp Neurol. 2013 Oct;248:170-82. doi: 
            10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20.
  PUBMED    23792206
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Neurol."; date: "2013-10"; volume: "248:170-82. doi"; pages: " 
            10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20"
COMMENT     Sequence Label: pLV-NT-3/D15A
FEATURES             Location/Qualifiers
     source          1..1467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(949..969)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1365..1376)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agcagagctc gtttagtgac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgactc tagaggatcc tacaggtgaa caagaccatg tccatcttgt
      121 tttatgtgat atttctcgct tatctccgtg gcatccaagg taacaacatg gatcaaagga
      181 gtttgccaga agactcgctc aattccctca ttattaagct gatccaggca gatattttga
      241 aaaacaagct ctccaagcag atggtggacg ttaaggaaaa ttaccagagc accctgccca
      301 aagctgaggc tccccgagag ccggagcggg gagggcccgc caagtcagca ttccagccgg
      361 tgattgcaat ggacaccgaa ctgctgcgac aacagagacg ctacaactca ccgcgggtcc
      421 tgctgagcga cagcaccccc ttggagcccc cgcccttgta tctcatggag gattacgtgg
      481 gcagccccgt ggtggcgaac agaacatcac ggcggaaacg gtacgcggag cataagagtc
      541 accgagggga gtactcggta tgtgccagtg aaagcttgtg ggtgaccgac aagtcatcgg
      601 ccatcgacat tcggggacac caggtcacgg tgctggggga gatcaaaacg ggcaactctc
      661 ccgtcaaaca atatttttat gaaacgcgat gtaaggaagc caggccggtc aaaaacggtt
      721 gcaggggtat tgatgataaa cactggaact ctcagtgcaa aacatcccaa acctacgtcc
      781 gagcactgac ttcagagaac aataaactcg tgggctggcg gtggatacgg atagacacgt
      841 cctgtgtgtg tgccttgtcg agaaaaatcg gaagaacatg aattggcatc tcgacaatca
      901 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
      961 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     1021 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     1081 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     1141 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     1201 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     1261 cactgacaat tccgtggngt tgtcggggaa nctgacgtcc tttccatggc tgctcgcctg
     1321 tgttgccacc tnnntctgcg cgggacgtcc ttctgctacg tccntcgncc ctcaatccag
     1381 cggannnnnn nccnnnnnnc tgcnntctgc gnnntnncgt ntcnnnnncc ncanannant
     1441 ngatnncctt ggnccnccnn ccccncc
//
