LOCUS       sequence-135519-        9365 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses the extracellular region of the PlexinC1 protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnc1-AP-His
FEATURES             Location/Qualifiers
     source          1..9365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5945..5962
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5988..6109
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6025..6044)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6079..6098
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6147..6164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6318..6906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6398..6417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7077..7868,7869..7937))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7700..7719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7938..8042)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8110..8128
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8166..8188)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8190..8210)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8195..8525
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8376..8511
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8438..8457
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8662..8681
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8748..9203)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8885..8906)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9097..9116
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9329..9351
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9343..9360
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9344..9360
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggaggtctc
     1621 ccggaggaag acgccgccgc gccccccgta ccccgccgca ccgttgcccc tgatcgccta
     1681 tctgctggcg ctggcagcgc ccgcccgggg cgcggacgag ccggtgtggc gatctgagca
     1741 agccatcgga gccatcgcgg cgagccgggc ggacggcgtg ttcgtggcga gcggcagctg
     1801 cctggaccag ctggactaca gcctgaagaa caggctctct cgcctatacc gggaccaagc
     1861 gggcaactgc acggagcccg tctcgttggc gccccccgcg cggccccggc ccgggagcag
     1921 cttcagcaag ctactgctgc cctaccgcga gggggcgacc ggcctcgagg ggctgctgct
     1981 caccggctgg accttcgacc ggggcgcctg cgaggtgcgg cccctgggca acctgaaccg
     2041 cagctccctg cgcaacggca ccgaggtggt gtcatgtcac ccgcagggtt cgacggctgg
     2101 tgtggtgtac cgcgcgagtg gcaccgacct ctggtacctg gcggtggccg ccacctatgt
     2161 gctgccggag ccggagaccg cgaaccgctg caaccccgca gcatccgatc gcgacacggc
     2221 tatcgcgctc aagaacacgg aggggcgcag tctggccacg caggagctgg gccgcctgaa
     2281 actgcgcggt agtgcgggca gcctgcactt cgtggacgcc tttctctgga acggcagtgt
     2341 ctacttcccc tactatccct acaactacac gagtggcgct gccaccggct ggcccagcat
     2401 ggcgcgcatc gcgcagagca cagaggtgct gttccagggc caggctgccc tcgactgcga
     2461 ccacggccac cctgagggcc gccgcctact gctttcctcc agcctggtgg aggctgtgga
     2521 catctgggcc ggcgtgttca gcgcggccac tggagagggc caggagaggc gctcccccgc
     2581 caccacggct ctctgcctct tcaggatgag tgagatccag gcgcacgcca ggagctgcag
     2641 ctgggacttt caagcgactg agcacaactg caaagaaggg gatcggcctg aacgggtcca
     2701 gccaattgca tcgtctacac tgatccactc tgacctgacg tctgtttatg gcaccgtggt
     2761 gatgaacagg actgttctgt tcttggggac cggagatggc cagttactga aggttgttct
     2821 tggtgagaat ttgacttcaa actgtccaga ggttatttat gaaattaaag aagaaacgcc
     2881 tgtgttctac aagctcgttc ctcatcccat gaagaacatc tacatctacc taacagctgg
     2941 gaaggaggtg agaagaattc ctgttgcaaa ctgcagtaaa cggaaatcct gttcagagtg
     3001 cttagcagcg gcagaccccc actgtggctg gtgcctcccg ttacaaaggt gcacttttca
     3061 aggggattgc acacatgctg ggagctttga aaactggctg gatatttcat caggacctaa
     3121 aaaatgcccg aaaattcaga tacttcgaag cctccgagag agaaccacag tgaccattgt
     3181 gggaagcatc tccgcaagac actcggagtg cgtggtgaag aacgcagaca cgggcaagct
     3241 gctctgccag gggagaagcc aactgaactg gacctgtgcc tgcaatatcc ccagcagacc
     3301 cagctataat gtcttagttg tcaatgcgac cttctccttt ccgtcttgga acttatcaga
     3361 aagattcaac ttcaccaact gtgcgtcatt aaaagaatgc ccggcgtgta tcagaagcgg
     3421 ctgtgcttgg tgtaaacggg acaagaaatg catccacccc ttcaccccct gcgaaccatc
     3481 agactatgag aggaatcagg agctctgtca agttgctgtg gagaagtcgc ccaaggactc
     3541 aggaggagga agagtcaagg agagcaagag aaacagaact gacggggctg tgcaggtctt
     3601 ctacattaag gccattgagc cacaaaaaat atcaacctta gggaaaagca atgtgattgt
     3661 tactggagca aacttcaccc aggcgtcaaa tatcaccatg atcctgcgag gaaccagtac
     3721 ctgcgagagg gatgtgatcc gggttagcca tgtgctaaat gacacgcata tgaaattctc
     3781 acttccatca agccgcaaag aaatgaaaga cgtttgcatc cagtttgacg gtgggacctg
     3841 ctcgtctgca ggggctctgt cctacattgc tctgccacat tgttccctga tagttcctgc
     3901 caccacctgg atcagcggtg ggcaaaacat aaccatcatg ggcagaaatt ttgacgtcat
     3961 cgacaacctc atcatttcac acgagctgaa gggaaacgca aatgtgaaca taaatgtctc
     4021 tgaatactgc gcagcaactt tctgcaggtt tctagctcct aatttaaaga gctccaaagt
     4081 acgtacaaac gtggccgtga agctgagggt ccaagacacc tacttggact gtggaacgct
     4141 gcaatacctc gaggacccca gatttacagg gtaccgagtg gagtcagaga ttgatacaga
     4201 attggaagta aaaattcaaa aagaaaatga caatttcaac atttccaagg atgacattga
     4261 tattactctc ttccatgggg aaaataaaca atttaattgc agttttgaaa atattactag
     4321 aaaccaggat ctcacaacca tcctttgcaa gatcaagagt atcaagaatg caaacaccat
     4381 cgctagctcc tctaaaaaag tccgcgtcaa gctgggaaac ctggaactct atgtggaaca
     4441 ggaatcagtt ccttccggcg cgcctggatc ttccggaatc atcccagttg aggaggagaa
     4501 cccggacttc tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc
     4561 tgcacagaca gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac
     4621 ggtgacagct gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc
     4681 cctggccatg gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca
     4741 tgtgccagac agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca
     4801 gaccattggc ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga
     4861 ggtcatctcc gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac
     4921 cacacgagtg cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg
     4981 gtactcggac gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac
     5041 gcagctcatc tccaacatgg acattgacgt gatcctaggt gggggccgaa agtacatgtt
     5101 tcgcatggga accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct
     5161 ggacgggaag aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtacgtgtg
     5221 gaaccgcact gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct
     5281 ctttgagcct ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct
     5341 gatggagatg acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct
     5401 cttcgtggag ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact
     5461 gactgagacg atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga
     5521 ggacacgctg agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc
     5581 cctgcgaggg agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta
     5641 cacggtcctc ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga
     5701 tgttaccgag agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga
     5761 cgaagagacc cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct
     5821 ggttcacggc gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct
     5881 ggagccctac accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc
     5941 gggtcatcat caccatcacc attaatctag agatcttatt aaagcagaac ttgtttattg
     6001 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6061 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt
     6121 cgactctagc tagactcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6181 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6241 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6301 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6361 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6421 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6481 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6541 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6601 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6661 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6721 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6781 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6841 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6901 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6961 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7021 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7081 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7141 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7201 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7261 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7321 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7381 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7441 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7501 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7561 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7621 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7681 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7741 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7801 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7861 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7921 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7981 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8041 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8101 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     8161 gaaaacctct gacacatgca gctcccggag gaatgtgtgt cagttagggt gtggaaagtc
     8221 cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8281 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8341 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8401 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8461 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8521 gcaaaaagct cccgggagct tgtatatcca ttttcgtccc ggagacggtc acagcttgtc
     8581 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     8641 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     8701 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt
     8761 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     8821 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     8881 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     8941 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     9001 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     9061 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     9121 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     9181 taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg
     9241 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     9301 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     9361 gaatt
//
