LOCUS       sequence-135743-        1091 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase
            with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Mullins EA, Kappock TJ
  TITLE     Crystal structures of Acetobacter aceti succinyl-coenzyme A 
            (CoA):acetate CoA-transferase reveal specificity determinants and 
            illustrate the mechanism used by class I CoA-transferases.
  JOURNAL   Biochemistry. 2012 Oct 23;51(42):8422-34. doi: 10.1021/bi300957f. 
            Epub 2012 Oct 11.
  PUBMED    23030530
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi300957f";
            journalName: "Biochemistry"; date: "2012-10-23- 23"; volume: "51"; 
            issue: "42"; pages: "8422-34"
COMMENT     Sequence Label: pJK514
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgatgat
       61 gggagcatgg tgcccgtatc aataaaccgc tgatgccaag acagagcttc cgtcagcaga
      121 tgcggtgtgt gcttaccaaa ggaattttta agcgcacgat caaaataatc ctgcaacatc
      181 ggccggtaat ccggatgcgc acatttggaa ataatttcac gcgcacgttg caccggtgaa
      241 agcccacgca gatccgccag cccctgcgcg gttacaaaaa tctgcgcatc ctgcatgatg
      301 tggtccacat gcgcagccat cggcacaatg gctgaaatct tgccaccttt ggctgtggag
      361 ggagacagga agatggacag gtaggaactc cgggcaaaat cacccgatcc cccaatacca
      421 ttcatcattt tggaacccat cacgcgggta gaattcacgt ttccgtaaat atccgcctca
      481 atcatgccgt tcatggcaat gcagcctaaa cggcggataa tgccggggct gttgctgaca
      541 tcctgctggc gcagaatgat cttgctgcgg aagaaatcca tccggttatt gatttcttcc
      601 gcagcttccg ggctcagcga gaaagacgag gcagaggcaa tacgcatacg gccagaatcc
      661 agcatggcca acatgccatc ctgaatcacc tcactatagc ccaccagatt ttcaaatggg
      721 ccttccttca gcccttccag cacggcgttg gccacattgc ccacaccaga ctggagcggc
      781 agaagtgagg gcggcaaacg gttctgtttg acttcatgcc caaagaaatc aagcaaatag
      841 cccgcaatag cttttgctgt ttcatccggc gcggcaaacg gggcatttct gtcccggtca
      901 ttcgtgcgta caatggcagc aatcttgtcc gggttcaccc gtaaaaccgg gccacccacg
      961 cgctgatctg cccgtacaat cggcacaata tcacgcgtag gcacgccact tacgttgcca
     1021 tcccagatat catggatgcc ttccagcatc ggattctgcc attcatttac ctcgataatg
     1081 accttttcgg c
//
