LOCUS       sequence-135822-        1092 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit (AaPurS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK533
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             320..342
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(373..392)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(374..392)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             901..1092
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttca ttccaccacc tctacagagt aatcttcaat caccaggttg gctagcagat
      121 cacgcgccat agcatcggcc tttttctggg cttcctcggc tttggcaccc ggcagatcca
      181 gttcaatcac cttacccacg cgcacatcgt gcacgccttc aaaaccaagt gtctgcaatg
      241 cgtggctgat ggccttgccc tgcgggtcca gaacaccgtc cttcagcatg acggttacac
      301 gtaccttcgc catggtatat ctccttctta aagttaaaca aaattatttc tagagggaaa
      361 ccgttgtggt ctccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgggcagc
      421 gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
      481 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
      541 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
      601 gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc
      661 atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg
      721 cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc
      781 tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc
      841 tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac ggaggcatca
      901 gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta
      961 acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg
     1021 cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt
     1081 gaaaacctct ga
//
