LOCUS       sequence-135878-        2558 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Sense Control Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL001
FEATURES             Location/Qualifiers
     source          1..2558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             44..73
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             89..106
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(162..179)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      332..378
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             520..1179
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(586..605)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1200..1294
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1701..1718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1835..2380)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1915..1934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaattctaaa gatcctaact cgagtaatga gaagcttggg cccgaacaaa aactcatctc
       61 agaagaggat ctgaatagcg ccgtcgacca tcatcatcat catcattgag tttaaacggt
      121 ctccagcttg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
      181 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
      241 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
      301 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      361 ctgggccttt cgttttatct gttgtttgtc ggtgaactaa tttctagact gcagttgatc
      421 gggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
      481 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
      541 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
      601 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
      661 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
      721 aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt
      781 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
      841 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
      901 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
      961 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     1021 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     1081 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     1141 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg
     1201 actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
     1261 cggttgccgc cgggcgtttt ttattggtga gaatccaagc ctccgatcaa cgtctcattt
     1321 tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc
     1381 tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc
     1441 caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta
     1501 tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg
     1561 gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag
     1621 tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga
     1681 tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
     1741 cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt
     1801 aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca
     1861 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
     1921 accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     1981 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
     2041 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc
     2101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca
     2161 ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta
     2221 aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt
     2281 caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt
     2341 ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca
     2401 gataaaatat ttctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag
     2461 ccccatacga tataagttgt aattctcatg tttgacagct tatcatcgat aagcttccga
     2521 tggcgcgccg agaggcttta cactttatgc ttccggct
//
