LOCUS       sequence-135887-        1032 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Anti-anti Sense Expression Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL1864
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        186..220
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(591..619)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc
       61 acctgaagtc agccccatac gatataagtt gtaattctca tgtttgacag cttatcatcg
      121 ataagcttcc gatggcgcgc cgagaggctt tacactttat gcttccggct gaattctaaa
      181 gatctttgac agctagctca gtcctaggta taatactagt aacaaaatcg actccttttt
      241 cctatgtcta gtccacatca gaaaaaggag tcgctcacgc cctgaccaaa gtttgtgaac
      301 gacatcattc aaagaaaaaa acactgagtt gtttttataa tcttgtatat ttagatatta
      361 aacgatattt aaatatacat aaagatatat atttgggtga gcgattcctt aaacgaaatt
      421 gagattaagg agtcgctctt ttttatgtat aaaaacaatc atgcaaatca ttcaaatcat
      481 ttggaaaatc acgatttaga caatttttct aaaaccggct actctaatag ccggttgtaa
      541 ggatctagga ggaaggatct atgagcaaag gagaagaact tttcactgga gttgtcccaa
      601 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg
      661 aaggtgatgc tacaaacgga aaactcaccc ttaaatttat ttgcactact ggaaaactac
      721 ctgttccgtg gccaacactt gtcactactc tgacctatgg tgttcaatgc ttttcccgtt
      781 atccggatca catgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac
      841 aggaacgcac tatatctttc aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt
      901 ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg tattgatttt aaagaagatg
      961 gaaacattct tggacacaaa ctcgagtaca actttaactc acacaatgta tacatcacgg
     1021 cagacaaaca aa
//
