LOCUS       sequence-135987-        1048 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit with N-terminal His6 tag (H6AaPurS); 
            glutaminase subunit (AaPurQ); large subunit (AaPurL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK625
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..94
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca
       61 tcatcacagc agcggcctgg tgccgcgcgg cagccatatg aaggtacgtg taaccgtcat
      121 gctgaaggac ggtgttctgg acccgcaggg caaggccatc agccacgcat tgcagacact
      181 tggttttgaa ggcgtgcacg atgtgcgcgt gggtaaggtg attgaactgg atctgccggg
      241 tgccaaagcc gaggaagccc agaaaaaggc cgatgccatg gcgcgtgatc tgctagccaa
      301 cctggtgatt gaagattact ctgtagaggt ggtggaatga aagcagcaat tatcgtattc
      361 cccggtacca accgggagcg cgatatggcc attgccctca agcgcgttac agggcaggcc
      421 ccgcgcatga tctggcacca tgaaacagat ctgggagata ccgatctagt cgtgctgcca
      481 ggtggtttca gctatggcga ttatctgcgc tgtggtgcca tggcagcgca ttctcccatc
      541 atggcggcta tcaaagcctt tgcccaaaag ggtggccata ttttgggtgt gtgcaacggc
      601 ttccagattt taacggaaac agggcttctg cccggtgctc tgttgcgcaa cgccaacctg
      661 cgctttctct ctcaggattg ctacctgaag gtggaagcag agaacacacc tttcactcac
      721 aagtggaaga agggtgatgt tttccgcacc cctctggccc atggtgatgg caattacacg
      781 gcagatatcg ccacgctgga acggcttgaa ggtgaaggtc ttgtggcttt ccactatgcc
      841 gatgccaaag gcaatgtgca ggctgatgat gccatcagca accccaatgg cagcatcaac
      901 agcattgcgg gtattctgag cgaaaacaaa cgcatttgcg gcatgatgcc tcatccagaa
      961 gatctggttg atcctctcat gggtggggaa gatggtctgc cattgttcca aggtctggtg
     1021 gaggctgtag tccggtgaat aagcctgt
//
