LOCUS       sequence-136013-         855 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of dmBACCS2NS, a modified Drosophila 
            melanogaster blue light-activated Ca2+ channel switch, along with 
            dOrai and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata 
            T
  TITLE     Light generation of intracellular Ca(2+) signals by a genetically 
            encoded protein BACCS.
  JOURNAL   Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021.
  PUBMED    26282514
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-08-18"; volume: "6:8021. doi"; pages: " 
            10.1038/ncomms9021"
COMMENT     Sequence Label: dmBACCS2NS-IRES-dOrai-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(78..788)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(356..375)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     625..643
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tctttcacaa attttgtaat ccagaggttg attatcgata agcttagatc ccgggcccgc
       61 ggtaccgtcg accgcggtta cttgtacagc tcgtccatgc cgccggtgga gtggcggccc
      121 tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac
      181 ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc
      241 ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc
      301 ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc
      361 ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg
      421 aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag
      481 ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg
      541 tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc
      601 caggcgaagg gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg
      661 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc
      721 atgtgcacct tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg
      781 ctcaccatat tatcatcgtg tttttcaaag gaaaaccacg tccccgtggt tcggggggcc
      841 tagacgtttt tttaa
//
