LOCUS       sequence-136825-        1034 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-MCS1-2A-MCS2
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(27..35,36..71,72..74,75..110,111..113,
                     114..149,150..155,156..773))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(774..827)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(908..928)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gatatcttgg atcgatcctt acttagttac ccggggagca tgtcaaggtc aaaatcgtca
       61 agagcgtcag caggcagcat atcaaggtca aagtcgtcaa gggcatcggc tgggagcatg
      121 tctaagtcaa aatcgtcaag ggcgtcggtc ggcccgccgc tttcgcactt tagctgtttc
      181 tccaggccac atatgattag ttccaggccg aaaaggaagg caggttcggc tccctgccgg
      241 tcgaacagct caattgcttg tctcagaagt gggggcatag aatcggtggt aggtgtctct
      301 ctttcctctt ttgctacttg atgctcctgt tcctccaata cgcagcccag tgtaaagtgg
      361 cccacggcgg acagagcgta cagtgcgttc tccagggaga agccttgctg acacaggaac
      421 gcgagctgat tttccagggt ttcgtactgt ttctctgttg ggcgggtgcc gagatgcact
      481 ttagccccgt cgcgatgtga gaggagagca cagcggtatg acttggcgtt gttccgcaga
      541 aagtcttgcc atgactcgcc ttccaggggg cagaagtggg tatgatgcct gtccagcatc
      601 tcgattggca gggcatcgag cagggcccgc ttgttcttca cgtgccagta cagggtaggc
      661 tgctcaactc ccagcttttg agcgagtttc cttgtcgtca ggccttcgat accgacacca
      721 ttgagtaatt ccagagctcc gtttatgact ttgctcttgt ccagtctaga cattggacca
      781 gggttttctt caacatcacc acaagtgagg agagaacctc taccttcggc accgggcttg
      841 cgggtcatgc accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag
      901 ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc
      961 ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc
     1021 tggtggtcgg gcga
//
