LOCUS       sequence-136874-         895 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  dCas9Master_mCBPHAT in pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1364-pmax-dCas9Master_mCBPHAT
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..27
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             108..134
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             138..158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgacaagttt gtacaaaaaa gcaggctccg aattcaccgg tgccgccacc atgatcgatg
       61 gtggcggtgg tagcggggga ggcggctccg ggggcggagg cagtatgtac ccatacgatg
      121 ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga agcgtccgac aagaagtaca
      181 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      241 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      301 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
      361 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
      421 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
      481 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
      541 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
      601 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
      661 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
      721 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
      781 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
      841 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg caacctgatt gccct
//
