LOCUS       sequence-136949-         889 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  dCas9Master_mCBPHAT in pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1364-pmax-dCas9Master_mCBPHAT
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    87..111
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      792..801
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgagaccgag gagagggtta gggataggct taccttcgaa ccgcgggccc tctagactcg
       61 agcggccgcc actgtgctgg atatcaacca ctttgtacaa gaaagctggg tcgaattctt
      121 aattaactag atgttgaggc agaagggcac agggcattta ttttcttggc agtgtttggc
      181 gtggtagcag caaagagcaa tgagctgctt gcacactggg catcctccat tagtcttgcg
      241 cttgcagccc ttggtgtgct gcacgactcg cttcatcttc tggcaagacg gcagtgagca
      301 gttggcattg cgacactggc aggcatgcac cagggactgg atgcagcgct ggatgctgag
      361 acgccgggat tcctgggggc tcttggactg tggctcaccc tgactgctgc cctcatcatc
      421 taggcctagc ccccacttca ccatcttatg ggtgtggctc tttgtgttgt agcaattgat
      481 acaaaggtca tagtcctcac acacagtgca gtgccagcgt gtttccacat ggtgtttgca
      541 ctcattgcag gtataaacaa agcggtcctg gccctgtgtg tgcagctcca ccagcatgca
      601 cagagtggac catttggagc ggcgtaagga agagaattcc cagtgcttgt ctctggccag
      661 ggtgaggaag gcatctcgcc catccatgag gtcacagcta agcagaggat cagggtccac
      721 gatggggggc tgagtgctga taacaggccc agcatgcaga tgaatcacaa agaatacctc
      781 cttgtgcttc tccatggtgg catacagctt ctgcgacagg tcgttggaaa cattgggcat
      841 gctgggcttc ttcttgttgg cgcggctaat gctgcttttg tttttgttg
//
