LOCUS       sequence-137163-        2444 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Oren R, Takahashi S, Doss C, Levy R, Levy S
  TITLE     TAPA-1, the target of an antiproliferative antibody, defines a new 
            family of transmembrane proteins.
  JOURNAL   Mol Cell Biol. 1990 Aug . 10(8):4007-15.
  PUBMED    1695320
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1990 Aug . 10(8):4007-15."
COMMENT     Sequence Label: pCDM8 hCD81
FEATURES             Location/Qualifiers
     source          1..2444
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..107
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     58..78
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     152..171
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        152..170
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      445..454
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          1863..1928
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2058..2078
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2350..2431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2387..2406)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
       61 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
      121 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
      181 cttctagaga tccctcgacc tcgagatcca ttgtgctgga aaggcgcgca acggcggcga
      241 cggcggcgac cccaccgcgc atcctgccag gcctccggcg cccagcgccc cacgcgcccc
      301 cgcgcccccg cgcccccgcg cccctttctt cgcgcccccg cccctcggcc cgccaggccc
      361 ccttgccggc cacccgccag gccccgcgcc ggcccgcccg ccgcccagga ccggcccgcg
      421 ccccgcaggc cgcccgccgc ccgcgccgcc atgggagtgg agggctgcac caagtgcatc
      481 aagtacctgc tcttcgtctt caatttcgtc ttctggctgg ctggaggcgt gatcctgggt
      541 gtggccctgt ggctccgcca tgacccgcag accaccaacc tcctgtatct ggagctggga
      601 gacaagcccg cgcccaacac cttctatgta ggcatctaca tcctcatcgc tgtgggcgct
      661 gtcatgatgt tcgttggctt cctgggctgc tacggggcca tccaggaatc ccagtgcctg
      721 ctggggacgt tcttcacctg cctggtcatc ctgtttgcct gtgaggtggc cgccggcatc
      781 tggggctttg tcaacaagga ccagatcgcc aaggatgtga agcagttcta tgaccaggcc
      841 ctacagcagg ccgtggtgga tgatgacgcc aacaacgcca aggctgtggt gaagaccttc
      901 cacgagacgc ttgactgctg tggctccagc acactgactg ctttgaccac ctcagtgctc
      961 aagaacaatt tgtgtccctc gggcagcaac atcatcagca acctcttcaa ggaggactgc
     1021 caccagaaga tcgatgacct cttctccggg aagctgtacc tcatcggcat tgctgccatc
     1081 gtggtcgctg tgatcatgat cttcgagatg atcctgagca tggtgctgtg ctgtggcatc
     1141 cggaacagct ccgtgtactg aggccccgca gctctggcca cagggacctc tgcagtgccc
     1201 cctaagtgac ccggacactt ccgagggggc catcaccgcc tgtgtatata acgtttccgg
     1261 tattactctg ctacacgtag cctttttact tttggggttt tgtttttgtt ctgaactttc
     1321 ctgttacctt ttcagggctg atgtcacatg taggtggcgt gtatgagtgg agacgggcct
     1381 gggtcttggg gactggaggg caggggtcct tctgccctgg ggtcccaggg tgctctgcct
     1441 gctcagccag gcctctcctg ggagccactc gcccagagac tcagcttggc caacttgggg
     1501 ggctgtgtcc acccagcccg cccgtcctgt gggctgcaca gctcaccttg ttccctcctg
     1561 ccccggttcg agagccgagt ctgtgggcac tctctgcctt catgcacctg tcctttctaa
     1621 cacgtcgcct tcaactgtaa tcacaacatc ctgactccgt catttaataa agaaggaaca
     1681 tcaggcatgc taaaaaaaaa aaaaaaactt tccagcacaa tggatctcga gggatcttcc
     1741 atacctacca gttctgcgcc tgcaggtcgc ggccgcgact ctagaggatc tttgtgaagg
     1801 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     1861 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     1921 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     1981 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     2041 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     2101 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     2161 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     2221 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
     2281 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     2341 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2401 aatttcacaa ataaagcatt tttttcactg cattctagtt gtgg
//
