LOCUS       sequence-137206-         826 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SHC1
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(658..678)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(703..720)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             731..753
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    768..792
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(792..811)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(793..811)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gtatccacct ttactgtcag cgcaactcga aggcctggcc
      121 aatggtgctg atgacatcct gggcaagccc ttcgggacac tccagaatgt ggcaggctct
      181 ctgattcaca gggtctttgg caacataggc gacatactcg gctgtgtccg gatccccgcc
      241 ggatgcaaat gagatagatt gcatgtggtg gttggcgatg atctgtttgc agtctgcggc
      301 catgaggttg aggctgctgg tggagacggt gagagtgatt ggcattccag caaatttcag
      361 gttactcctc cccaggatag agctgagcgg gcggctacag ggctttctcc tccttgtcgc
      421 ccccttagca cccggcacag cctcacacac cagactgatg gcctccctgg tgacctgagt
      481 ccgggtgttg aagtccaggg cacgcattga ctggaggacc tccacacaac ccatgtaccg
      541 aaccaagtag gaaaccccgg gtcccatgac tttgtcgttg ggatgcagcc agccccgcgt
      601 gggcttattg acaaagctcc cgtggcgggt ccactcctcg cccccaagct ggcccatgga
      661 ttggaagtac aggttctcgg tacccagatc tacaccagaa gaatgatgat gatgatggtg
      721 catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga attgttatcc
      781 gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcga
//
