LOCUS       sequence-137214-         857 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP9
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(669..689)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(714..731)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             742..764
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    779..803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(803..822)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(804..822)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atccgtatcc acctttactg tcagttcaaa
      121 ctcagcatct tggacctctt tgccatttca gaactgaaag ccttatatat ctccaaatct
      181 gttggaaaga tcacaaattt tacagttaac tggtgtttta catggtcttt ggcaaatgtt
      241 aaaacttcat caaacaaaat ctctgctgct gtttccttct ttatttccat gtttccagtc
      301 ccaagggcag gaaaggaaat ggaagttata ttttgctcaa tgcatttttc caaacactcc
      361 ttcattgcat gttttaatat ctgaggttta ggaaattctg aatgccacag tacatggtat
      421 atatatttac agaacaagtt aaatcctttt gtgaccagta ccaactggga ccgttgaaac
      481 tgtttagcct ttgtggcaag aaattccgat ttcatttcaa ctcctgcttg ttgtagaatt
      541 gactttgcca caggtccaac tgtaatatca tgtgggttta cagaattaac aattacatct
      601 gccgtctgcc attcaatgtg gccctggaca atctggaggg tcaggttgtt cacgaccatt
      661 gcattcatgg attggaagta caggttctcg gtacccagat ctacaccaga agaatgatga
      721 tgatgatggt gcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg
      781 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
      841 ctcgatcctc tacgccg
//
