LOCUS       sequence-137255-         936 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression of Maltose Binding Protein linked to SnoopTag, 
            a peptide tag forming a spontaneous covalent bond with its protein 
            partner Snoopcatcher..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, 
            Howarth M
  TITLE     Programmable polyproteams built using twin peptide superglues.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
  PUBMED    26787909
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 19. pii: 201519214."
COMMENT     Sequence Label: pET28a SnoopTag-MBP
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(74..97)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttttacg
       61 agctcgaatt agtctgcgcg tctttcaggg cttcatcgac agtctgacga ccgctggcgg
      121 cgttgatcac cgcagtacgc acggcatacc agaaagcgga catctgcggg atgttcggca
      181 tgatttcacc tttctgggcg ttttccatag tggcggcaat acgtggatct ttcgccaact
      241 cttcctcgta agacttcagc gctacggcac ccagcggttt gtctttatta accgcttcca
      301 gaccttcatc agtcagcaga tagttttcga ggaactcttt tgccagctct ttgttcggac
      361 tggcggcgtt aatacctgcg ctcagcacgc caacgaacgg tttggatggt tgacccttga
      421 aggtcggcag taccgttaca ccataattca ctttgctggt gtcgatgttg gaccatgccc
      481 acgggccgtt gatggtcatc gctgtttcgc ctttattaaa ggcagcttct gcgatggagt
      541 aatcggtgtc tgcattcatg tgtttgtttt taatcaggtc aaccaggaag gtcagacccg
      601 ctttcgcgcc agcgttatcc acgcccacgt ctttaatgtc gtacttgccg ttttcatact
      661 tgaacgcata acccccgtca gcagcaatca gcggccaggt gaagtacggt tcttgcaggt
      721 tgaacatcag cgcgctctta cctttcgctt tcagttcttt atccagcgcc gggatctctt
      781 cccaggtttt tggcgggttc ggcagcagat ctttgttata aatcagcgat aacgcttcaa
      841 cagcgatcgg gtaagcaatc agcttgccgt tgtaacgtac ggcatcccag gtaaacggat
      901 acagcttgtc ctggaacgct ttgtccgggg tgattt
//
