LOCUS       sequence-137286-         582 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses BE1 with N-terminal His6 tag in E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR
  TITLE     Programmable editing of a target base in genomic DNA without 
            double-stranded DNA cleavage.
  JOURNAL   Nature. 2016 Apr 20. doi: 10.1038/nature17946.
  PUBMED    27096365
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Apr 20. doi: 10.1038/nature17946."
COMMENT     Sequence Label: pET28b-BE1
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             142..159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
       61 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacca tgggcagcag ccatcatcat catcatcaca tgtcttctga aaccggtccg
      181 gttgcggttg acccgaccct gcgtcgtcgt atcgaaccgc acgaattcga agttttcttc
      241 gacccgcgtg aactgcgtaa agaaacctgc ctgctgtacg aaatcaactg gggtggtcgt
      301 cactctatct ggcgtcacac ctctcagaac accaacaaac acgttgaagt taacttcatc
      361 gaaaaattca ccaccgaacg ttacttctgc ccgaacaccc gttgctctat cacctggttc
      421 ctgtcttggt ctccgtgcgg tgaatgctct cgtgcgatca ccgaattcct gtctcgttac
      481 ccgcacgtta ccctgttcat ctacatcgcg cgtctgtacc accacgcgga cccgcgtaac
      541 cgtcagggtc tgcgtgacct gatctcttct ggtgttacca tc
//
