LOCUS       sequence-137378-        8714 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses the extracellular region of the Contactin 1 protein 
            (truncated immediately upstream of propeptide cleavage and 
            GPI-attachement site), C-terminally fused to the Fc region of human 
            IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Cntn1-Fc-His
FEATURES             Location/Qualifiers
     source          1..8714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             5294..5311
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5337..5458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5374..5393)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5428..5447
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5496..5513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5667..6255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5747..5766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6426..7217,7218..7286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7049..7068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7287..7391)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7459..7477
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7515..7537)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7539..7559)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7544..7874
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7725..7860
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7787..7806
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8011..8030
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8097..8552)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8234..8255)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8446..8465
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8678..8700
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8692..8709
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8693..8709
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgaaaatgcc
     1621 attgctggtc agccatctcc tgctaatctc cttaacatct tgtctaggag actttacctg
     1681 gcacagaaga tatggtcatg gagtgtctga ggaggacaaa ggatttgggc ccatttttga
     1741 agagcagcca atcaatacca tttacccaga agagtcactg gaaggaaaag tgtcactcaa
     1801 ctgccgggca cgcgccagcc catttccagt ttacaaatgg agaatgaata acggggacgt
     1861 ggacctgaca aacgatcggt acagtatggt cggaggaaac cttgttatca acaacccgga
     1921 caaacagaaa gacgctgggg tgtactactg cctggcatcc aacaactacg gaatggtcag
     1981 gagcacggag gcaactctga gcttcgggta tctcgacccc tttcctcccg aggagcgtcc
     2041 ggaagtgaag gtgaaggaag ggaagggcat ggtgcttctc tgtgatcctc cctaccactt
     2101 cccagatgat cttagctacc gctggcttct gaatgaattt cctgtattta tcaccatgga
     2161 taagcgaaga tttgtgtctc agaccaacgg caatctctat atcgcaaatg tcgagtcttc
     2221 tgacagaggc aactactcct gctttgtgtc cagtccttct attacaaaga gtgtgttcag
     2281 caagtttatt cccctcattc cgattcctga acgaacaaca aagccatatc ctgctgatat
     2341 tgtggtgcag ttcaaggaca tatacaccat gatgggccaa aatgtgactt tagagtgttt
     2401 cgctctaggg aaccctgttc ctgatatccg gtggcggaag gtgctagaac caatgcccag
     2461 cactgctgag ataagcacgt caggggctgt cctcaagatc ttcaacatcc agctagaaga
     2521 tgaaggcctc tacgagtgtg aggctgagaa catccgggga aaggacaagc accaggcaag
     2581 aatctatgtt caagcgtttc ctgagtgggt agagcatatc aatgacactg aggtggacat
     2641 aggcagtgac ctctactggc cttgcatagc cacagggaag cccattccta ccatcaggtg
     2701 gctgaaaaat ggatactcgt atcacaaagg ggaactgaga ctgtatgatg tgacttttga
     2761 aaatgccggg atgtaccagt gcatagcaga aaacgcgtat gggtccattt atgccaacgc
     2821 tgagctcaag atcctggctt tagctccaac ttttgaaatg aatcctatga agaaaaagat
     2881 cttggctgcg aaaggtggaa gggttatcat tgaatgtaaa cccaaggccg caccaaaacc
     2941 caagttttca tggagcaaag ggacggagtg gctcgttaac agcagcagaa tactcatatg
     3001 ggaagatggt agcttggaaa tcaataatat tacaagaaat gatggtggaa tctatacatg
     3061 ctttgcagaa aataacagag gaaaagctaa tagcactggg actctggtga tcacaaatcc
     3121 cacacgaatt attttggccc caatcaatgc tgacatcact gttggggaaa atgccaccat
     3181 gcagtgtgct gcgtctttcg atcctgcctt ggacctcacg tttgtgtggt ccttcaatgg
     3241 ctacgtgatt gactttaaca aggaaattac gcatatccat taccagagga attttatgct
     3301 cgacgccaat ggggaactgc tcatccgaaa tgcgcagctg aaacacgcag gccggtacac
     3361 gtgcacggcg cagacaatcg tggacaattc ctcggcttcc gctgaccttg tcgttcgagg
     3421 ccccccaggt cccccaggag gcctgagaat agaagacatc agagctacct ctgtggcact
     3481 gacttggagc cgtggttccg acaatcacag ccctatctct aaatacacga tccagaccaa
     3541 gacgattctc tcagatgact ggaaagatgc gaagacagat cccccaatca ttgaaggaaa
     3601 catggagtca gcaaaagcag tggatttgat cccttggatg gagtatgaat ttcgagtggt
     3661 ggcgaccaat actttaggga caggagagcc cagcataccc tcaaacagga tcaaaacaga
     3721 cggtgctgca ccaaatgtgg ctccctcaga tgtaggcggt gggggcggaa ccaacagaga
     3781 gctgacaata acgtgggcgc ctttgtcgag agagtaccat tacggtaaca attttggtta
     3841 catagtggcg tttaagccat ttgatggaga agagtggaag aaagtgactg tgactaaccc
     3901 agacactggc cgctacgtcc acaaagatga gaccatgacc ccctccaccg cgtttcaagt
     3961 caaagtgaaa gccttcaaca ataaaggaga tgggccctac agcctcgtag ctgtcatcaa
     4021 ctctgcacaa gatgctccca gtgaagcccc cacagaggta ggggtcaaag tgttatcatc
     4081 ctctgagata tctgtccatt ggaagcatgt gttagagaaa attgtggaaa gctatcagat
     4141 tcggtactgg gctgggcatg acaaagaagc agccgcacac agagttcagg tcaccagcca
     4201 ggagtactca gccagactgg agaaccttct tcccgacacc cagtacttca ttgaagtggg
     4261 agcctgcaac agtgccggat gcggaccttc cagtgatgtg atcgagacct tcaccagaaa
     4321 agcccctcct agccaaccac caaggatcat cagttcggtg agatctggct cacgatatat
     4381 aatcacatgg gatcacgttg tggcactatc caatgaatct actgtgacag gctacaagat
     4441 actctataga ccggatggcc aacatgacgg caagctgttc tcaacccata aacactccat
     4501 agaagtcccc atccccagag acggagagta tgttgtcgag gttcgagcac acagcgacgg
     4561 gggagacgga gtcgtgtctc aagtcaaaat ttcaggcgtg actagtgaca aaactcacac
     4621 atgcccaccg tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc
     4681 aaaacccaag gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga
     4741 cgtgagccac gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca
     4801 taatgccaag acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt
     4861 cctcaccgtc ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa
     4921 caaagccctc ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga
     4981 accacaggtg tataccctgc ccccatcccg ggatgagctg accaagaacc aggtcagcct
     5041 gacctgcctg gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg
     5101 gcagccggag aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt
     5161 cctctacagc aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg
     5221 ctccgtgatg catgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc
     5281 gggtaaaggc ggccatcatc accatcacca ttaatctaga gatcttatta aagcagaact
     5341 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5401 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5461 atgtctggtc gactctagct agactcttcc gcttcctcgc tcactgactc gctgcgctcg
     5521 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5581 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5641 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5701 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5761 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5821 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5881 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5941 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6001 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6061 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6121 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6181 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6241 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6301 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6361 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6421 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6481 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6541 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6601 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6661 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6721 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6781 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6841 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6901 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6961 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7021 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7081 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7141 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7201 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7261 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7321 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7381 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     7441 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     7501 gatgacggtg aaaacctctg acacatgcag ctcccggagg aatgtgtgtc agttagggtg
     7561 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     7621 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7681 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7741 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7801 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7861 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcgtcccg gagacggtca
     7921 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     7981 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8041 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     8101 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     8161 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     8221 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     8281 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     8341 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     8401 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     8461 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     8521 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     8581 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     8641 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     8701 acggccagtg aatt
//
