LOCUS       sequence-137419-        2166 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Xenopus oocyte expression vector containing the cDNA of human PepT1 
            with a c terminal flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Beale JH, Parker JL, Samsudin F, Barrett AL, Senan A, Bird LE, Scott
            D, Owens RJ, Sansom MS, Tucker SJ, Meredith D, Fowler PW, Newstead S
  TITLE     Crystal Structures of the Extracellular Domain from PepT1 and PepT2 
            Provide Novel Insights into Mammalian Peptide Transport.
  JOURNAL   Structure. 2015 Oct 6;23(10):1889-99. doi: 
            10.1016/j.str.2015.07.016. Epub 2015 Aug 27.
  PUBMED    26320580
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.str.2015.07.016"; journalName: "Structure"; date: 
            "2015-10-6- 6"; volume: "23"; issue: "10"; pages: "1889-99"
COMMENT     Sequence Label: pBF_HsPepT1
FEATURES             Location/Qualifiers
     source          1..2166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2139..2162
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gatccgccgc caccatggga atgtccaaat cacacagttt ctttggttat cccctgagca
       61 tcttcttcat cgtggtcaat gagttttgcg aaagattttc ctactatgga atgcgagcaa
      121 tcctgattct gtacttcaca aatttcatca gctgggatga taacctgtcc accgccatct
      181 accatacgtt tgtggctctg tgctacctga cgccaattct cggagctctt atcgccgact
      241 cgtggctggg aaagttcaag accattgtgt cgctctccat tgtctacaca attggacaag
      301 cagtcacctc agtaagctcc attaatgacc tcacagacca caaccatgat ggcacccccg
      361 acagccttcc tgtgcacgtg gtgctgtcct tgatcggcct ggccctgata gctctcggga
      421 ctggaggaat caaaccctgt gtgtctgcgt ttggtggaga tcagtttgaa gagggccagg
      481 agaaacaaag aaacagattt ttttccatct tttacttggc tattaatgct ggaagtttgc
      541 tttccacaat catcacaccc atgctcagag ttcaacaatg tggaattcac agtaaacaag
      601 cttgttaccc actggccttt ggggttcctg ctgctctcat ggctgtagcc ctgattgtgt
      661 ttgtccttgg cagtgggatg tacaagaagt tcaagccaca gggcaacatc atgggtaaag
      721 tggccaagtg catcggtttt gccatcaaaa atagatttag gcatcggagt aaggcatttc
      781 ccaagaggga gcactggctg gactgggcta aagagaaata cgatgagcgg ctcatctccc
      841 aaattaagat ggttacgagg gtgatgttcc tgtatattcc actcccaatg ttctgggcct
      901 tgtttgacca gcagggctcc aggtggacac tgcaggcaac aactatgtcc gggaaaatcg
      961 gagctcttga aattcagccc gatcagatgc agaccgtgaa cgccatcctg atcgtgatca
     1021 tggtcccgat cttcgatgct gtgctgtacc ctctcattgc aaaatgtggc ttcaatttca
     1081 cctccttgaa gaagatggca gttggcatgg tcctggcctc catggccttt gtggtggctg
     1141 ccatcgtgca ggtggaaatc gataaaactc ttccagtctt ccccaaagga aacgaagtcc
     1201 aaattaaagt tttgaatata ggaaacaata ccatgaatat atctcttcct ggagagatgg
     1261 tgacacttgg cccaatgtct caaacaaatg catttatgac ttttgatgta aacaaactga
     1321 caaggataaa catttcttct cctggatcac cagtcactgc cgtaactgac gacttcaagc
     1381 agggccaacg ccacacgctt ctagtgtggg cccccaatca ctaccaggtg gtaaaggatg
     1441 gtcttaacca gaagccagaa aaaggggaaa atggaatcag atttgtaaat acttttaacg
     1501 agctcatcac catcacaatg agtgggaaag tttatgcaaa catcagcagc tacaatgcca
     1561 gcacatacca gttttttcct tctggcataa aaggcttcac aataagctca acagagattc
     1621 cgccacaatg tcaacctaat ttcaatactt tctaccttga atttggtagt gcttatacct
     1681 atatagtcca aaggaagaat gacagctgcc ctgaagtgaa ggtgtttgaa gatatttcag
     1741 ccaacacagt taacatggct ctgcaaatcc cgcagtattt tcttctcacc tgtggcgaag
     1801 tggtcttctc tgtcacggga ttggaattct catattctca ggctccttcc aacatgaagt
     1861 cggtgcttca ggcaggatgg ctgctgaccg tggctgttgg caacatcatt gtgctcatcg
     1921 tggcaggggc aggccagttc agcaaacagt gggccgagta cattctattt gccgcgttgc
     1981 ttctggtcgt ctgtgtaatt tttgccatca tggctcggtt ctatacttac atcaacccag
     2041 cggagatcga agctcaattt gatgaggatg aaaagaaaaa cagactggaa aagagtaacc
     2101 catatttcat gtcaggggcc aattcacaga aacagatgga ctataaggac gacgatgaca
     2161 agtgaa
//
