LOCUS       sequence-137457-         795 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  expression vector designed for expression, secretion, and display of
            proteins on the extracellular surface of Saccharomyces cerevisiae 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Kieke MC, Cho BK, Boder ET, Kranz DM, Wittrup KD
  TITLE     Isolation of anti-T cell receptor scFv mutants by yeast surface 
            display.
  JOURNAL   Protein Eng. 1997 Nov;10(11):1303-10.
  PUBMED    9514119
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Eng."; date: "1997-11"; volume: "10(11)"; pages: "1303-10"
COMMENT     Sequence Label: pYD1
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             384..413
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             join(417..425,426..440)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             519..560
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             570..587
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atacgactca ctatagggaa tattaagcta attccctact tcatacattt tcaattaaga
       61 tgcagttact tcgctgtttt tcaatatttt ctgttattgc ttcagtttta gcacaggaac
      121 tgacaactat atgcgagcaa atcccctcac caactttaga atcgacgccg tactctttgt
      181 caacgactac tattttggcc aacgggaagg caatgcaagg agtttttgaa tattacaaat
      241 cagtaacgtt tgtcagtaat tgcggttctc acccctcaac gactagcaaa ggcagcccca
      301 taaacacaca gtatgttttt aagcttctgc aggctagtgg tggtggtggt tctggtggtg
      361 gtggttctgg tggtggtggt tctgctagca tgactggtgg acagcaaatg ggtcgggatc
      421 tgtacgacga tgacgataag gtaccaggat ccagtgtggt ggaattctgc agatatccag
      481 cacagtggcg gccgctcgag tctagagggc ccttcgaagg taagcctatc cctaaccctc
      541 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
      601 gctgatctga taacaacagt gtagatgtaa caaaatcgac tttgttccca ctgtactttt
      661 agctcgtaca aaatacaata tacttttcat ttctccgtaa acaacatgtt ttcccatgta
      721 atatcctttt ctatttttcg ttccgttacc aactttacac atactttata tagctattca
      781 cttctataca ctaaa
//
