LOCUS       sequence-137461-        3951 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  STAG2 cDNA (CCDS43990) S653X.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Kim JS, He X, Orr B, Wutz G, Hill V, Peters JM, Compton DA, Waldman 
            T
  TITLE     Intact Cohesion, Anaphase, and Chromosome Segregation in Human Cells
            Harboring Tumor-Derived Mutations in STAG2.
  JOURNAL   PLoS Genet. 2016 Feb 12;12(2):e1005865. doi: 
            10.1371/journal.pgen.1005865. eCollection 2016 Feb.
  PUBMED    26871722
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2016-02-12"; volume: "12(2):e1005865. doi"; pages: "
            10.1371/journal.pgen.1005865. eCollection 2016 Feb"
COMMENT     Sequence Label: pcDNA3.1-FLAG-SBP-STAG2 (S653X)
FEATURES             Location/Qualifiers
     source          1..3951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             28..141
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 atggactaca aggacgacga cgacaagatg gacgagaaga ccaccggctg gcgcggcggc
       61 cacgtggtgg agggcctggc cggcgagctg gagcagctgc gcgcccgcct ggagcaccac
      121 ccccagggcc agcgcgagcc cggcggcata gcagctccag aaataccaac tgattttaat
      181 ctactacagg agtcagaaac acatttttct tctgacacag attttgaaga tatcgaagga
      241 aaaaaccaaa agcaaggcaa aggcaaaact tgtaaaaaag gcaaaaaggg cccagcagaa
      301 aagggcaaag gtggaaatgg aggaggaaaa cctccttctg gtccaaaccg aatgaatggt
      361 catcaccaac agaatggagt ggaaaacatg atgttgtttg aagttgttaa aatgggcaag
      421 agtgctatgc agtcggtggt agatgattgg atagaatcat acaagcatga ccgagatata
      481 gcacttcttg accttatcaa cttttttatt cagtgttcag gctgtaaagg agttgtcaca
      541 gcagaaatgt ttagacatat gcagaactct gagataattc gaaaaatgac tgaagagttc
      601 gatgaggata gtggagatta tccacttacc atggctggtc ctcagtggaa gaagttcaaa
      661 tccagttttt gtgagttcat tggcgtgtta gtacggcaat gtcaatatag tatcatatat
      721 gatgagtata tgatggatac agtcatttca cttcttacag gattgtctga ctcacaagtc
      781 agagcatttc gacatacaag caccctggca gctatgaagt tgatgacagc tttggtgaat
      841 gtggcactaa atcttagcat taatatggat aatacacaaa gacaatatga agcagaacgg
      901 aataaaatga ttggaaaacg agccaatgag aggctagaac tcctgctaca aaagcggaaa
      961 gagcttcagg aaaatcaaga tgaaatagaa aatatgatga atgcaatatt taaaggagtg
     1021 tttgtacata gataccgtga tgcgatagct gaaattcgag ctatttgcat tgaagagatt
     1081 ggcatttgga tgaagatgta tagtgatgcc tttcttaatg acagttattt aaaatatgtt
     1141 ggttggacta tgcatgataa gcaaggtgaa gtaagactca aatgtcttac tgctctacaa
     1201 gggctttatt ataacaaaga gcttaattcc aaactggaac tttttaccag tcggttcaag
     1261 gatagaattg tgtctatgac ccttgacaaa gaatatgatg ttgcagtaca agcaataaaa
     1321 ttactcactc ttgttttaca gagtagtgaa gaagttctca ctgcagaaga ttgtgaaaat
     1381 gtctatcatc tggtttattc agctcaccgg ccagtagcag tagcagctgg agaatttctc
     1441 tacaaaaagc tcttcagtcg tagagatcca gaggaggatg gaatgatgaa aagaagagga
     1501 agacaaggtc caaatgccaa ccttgttaag acattggttt ttttctttct agaaagtgag
     1561 ttacatgagc atgcagcata ccttgtggat agcatgtggg actgtgctac tgagctgctg
     1621 aaagactggg aatgtatgaa tagcttgtta ctggaagagc cacttagtgg agaggaagca
     1681 ctaacagata ggcaagagag tgctctgatt gaaataatgc tttgtaccat tagacaagcg
     1741 gctgaatgtc atcctcccgt gggaagaggg acaggaaaaa gggtgcttac agcaaaggag
     1801 aagaagacac agttggatga taggacaaaa atcactgagc tttttgccgt ggcccttcct
     1861 cagttattag caaaatactc tgtagatgca gaaaaggtga ctaacttgtt gcagttgcct
     1921 cagtactttg atttggaaat atataccact ggacgattag aaaagcattt ggatgcctta
     1981 ttgcgacaga tccggaatat tgtagagaag cacacagata cagatgtttt ggaagcatgt
     2041 tctaaaactt accatgcact ctgtaatgaa gagttcacaa tcttcaacag agtagatatt
     2101 tgaagaagtc aactgataga tgaattggca gataaattta accggcttct tgaagatttt
     2161 ctgcaagagg gtgaagaacc tgatgaagat gatgcatatc aggtattgtc aacattgaag
     2221 aggatcactg cttttcataa tgcccatgac ctttcaaagt gggatttatt tgcttgtaat
     2281 tacaaactct tgaaaactgg aatcgaaaat ggagacatgc ctgagcagat tgttattcac
     2341 gcactgcagt gtactcacta tgtaatcctt tggcaacttg ctaagataac tgaaagcagc
     2401 tctacaaagg aggacttgct gcgtttaaag aaacaaatga gagtattttg tcagatatgt
     2461 caacattacc tgaccaacgt gaatactact gttaaggaac aggccttcac tattctgtgt
     2521 gatattttga tgatcttcag ccatcagatt atgtcaggag ggcgtgacat gttagagcca
     2581 ttagtgtata cccctgattc ttcattgcag tctgagttgc tcagctttat tttggatcat
     2641 gtcttcattg aacaggatga tgataataat agtgcagatg gtcagcaaga ggatgaagcc
     2701 agtaaaattg aagctctgca caagagaaga aatttacttg cagcattttg taagctaatt
     2761 gtatatactg tggtggagat gaatacagct gcagatatct tcaaacagta tatgaagtat
     2821 tataatgact atggagatat catcaaagaa acaatgagta aaacaaggca gatagacaaa
     2881 attcagtgtg ctaagaccct tattctcagt ctgcaacagc tttttaatga aatgatacaa
     2941 gaaaatggct ataattttga tagatcatcc tctacattta gtggcataaa agaacttgct
     3001 cgacgttttg ctttaacttt tggacttgat cagttgaaaa caagagaagc cattgccatg
     3061 ctacacaaag atggcataga atttgctttt aaagagccta atccgcaagg ggagagccat
     3121 ccacctttaa atttggcatt tcttgatatt ctgagtgaat tttcttctaa actacttcga
     3181 caagacaaaa gaacagtgta tgtttacttg gaaaagttca tgacctttca gatgtcactc
     3241 cgaagagagg atgtgtggct tccactgatg tcttaccgaa attctttgct agctggtggt
     3301 gatgatgaca ccatgtcagt cattagtgga atcagcagcc gggggtcaac agtacggagt
     3361 aaaaaatcaa aaccatctac aggaaaacgg aaagtggttg agggcatgca gctttcactc
     3421 actgaagaaa gtagtagtag tgacagtatg tggttaagca gagaacaaac actgcacacc
     3481 cctgttatga tgcagacacc acaactcacc tccactatta tgagagagcc caaaagatta
     3541 cggcctgagg atagcttcat gagtgtttat ccaatgcaga ctgaacatca tcaaacacct
     3601 cttgattata acacgcaggt aacatggatg ttagctcaaa gacaacaaga ggaagcaagg
     3661 caacagcagg agagagcagc aatgagctat gttaaactgc gaactaatct tcagcatgcc
     3721 attcggcgtg gcacaagcct aatggaagat gatgaagagc caattgtgga agatgttatg
     3781 atgtcctcag aagggaggat tgaggatctt aatgagggaa tggattttga caccatggat
     3841 atagatttgc caccatcaaa gaacagacga gagagaacag aactgaagcc tgatttcttt
     3901 gatccagctt caattatgga tgaatcagtt cttggagtgt caatgtttta a
//
