LOCUS       sequence-137512-        9621 bp    DNA     circular SYN 15-APR-2025
DEFINITION  mYPet_PH pCFJ150 - contain C. elegans codon optimized mYPet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Heppert JK, Dickinson DJ, Pani AM, Higgins CD, Steward A, Ahringer 
            J, Kuhn JR, Goldstein B
  TITLE     Comparative assessment of fluorescent proteins for in vivo imaging 
            in an animal model system.
  JOURNAL   Mol Biol Cell. 2016 Nov 7;27(22):3385-3394. doi: 
            10.1091/mbc.E16-01-0063. Epub 2016 Jul 6.
  PUBMED    27385332
REFERENCE   2  (bases 1 to 9621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E16-01-0063"; journalName: "Mol Biol Cell"; date: 
            "2016-11-7- 7"; volume: "27"; issue: "22"; pages: "3385-3394"
COMMENT     Sequence Label: JH05
FEATURES             Location/Qualifiers
     source          1..9621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             1944..1967
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1983..2006
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      2018..2341
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(3920..3939)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4039..4061
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4099..4117)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4185..4289
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4290..4358,4359..5150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4508..4527)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5321..5909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5810..5829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7545..7556)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 caactttgta tagaaaagtt gaaatatcag tttttaaaaa attaaaccat aaaacaaata
       61 atataaccca atttttacat caaaccacaa gaaaaaaata catttgggcc cacggataaa
      121 gaaattaaaa aaatacattt tttaaaggcg caccgaatta aaattcattt gggtcttacc
      181 gcgtataccg tactccgttt gtttgatcat ttttgtcagc gctggcggtt gttttttcat
      241 ttcatttctg cttcaaagac gttttctcga ataatttttc gtttattctc ttttttaaaa
      301 ttaatttcta gccgtaaatg ttataaattc acccatttaa cgcaaatttc atggtaatct
      361 catggaaaaa tgcagtttct ttgttaaaga aagcttaaat agcaaaaatt ccccgacttt
      421 ccccaaaatc ctgctcgatt ttccgttttc tcattgtatt ctctcttaat taattttatc
      481 gataatcaat tgaatgtttc agacagagaa tgtcaaaagg agaagagtta tttacaggag
      541 ttgtacctat cctcgtcgag ctcgacggag acgtcaacgg acacaagttc tccgtctccg
      601 gagagggaga gggagacgcc acctacggaa agctcaccct caagctcctc tgcaccaccg
      661 gaaagctccc agtcccatgg ccaaccctcg tcaccaccct cggatacgga gtccaatgct
      721 tcgcccgtta cccagaccac atgaagcaac acgacttctt caagtccgcc atgccagagg
      781 gatacgtcca agagcgtacc atcttcttca aggtaagttt aaacatatat atactaacta
      841 accctgatta tttaaatttt caggacgacg gaaactacaa gacccgtgcc gaggtcaagt
      901 tcgagggaga caccctcgtc aaccgtatcg agctcaaggt aagtttaaac agttcggtac
      961 taactaacca tacatattta aattttcagg gaatcgactt caaggaggac ggaaacatcc
     1021 tcggacacaa gcttgagtac aactacaact cccacaacgt ctacatcacc gccgacaagc
     1081 aaaagaacgg aatcaaggcc aacttcaagg taagtttaaa catgatttta ctaactaact
     1141 aatctgattt aaattttcag atccgtcaca acatcgagga cggaggagtc caactcgccg
     1201 accactacca acaaaacacc ccaatcggag acggaccagt cctcctccca gacaaccact
     1261 acctctccta ccaatccaag ctcttcaagg acccaaacga gaagcgtgac cacatggtcc
     1321 tccttgagtt cctcaccgcc gccggaatca ccgagggaat gaacgagctc tacaaggccg
     1381 gccacggact ccaagacgac ccagacctcc aagccctcct caagggatcc caactcctca
     1441 aggtcaagtc ctcctcctgg cgtcgtgagc gtttctacaa gctccaagag gactgcaagg
     1501 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag accatctggc
     1561 aagagtcccg taaggtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
     1621 ttcaggtcat gcgttcccca gagtcccaac tcttctccat cgaggacatc caagaggtcc
     1681 gtatgggaca ccgtaccgag ggactcgaga agttcgcccg tgacatccca gaggaccgtt
     1741 gcttctccat cgtcttcaag gtaagtttaa acatgatttt actaactaac taatctgatt
     1801 taaattttca ggaccaacgt aacaccctcg acctcatcgc cccatcccca gccgacgccc
     1861 aacactgggt ccaaggactc cgtaagatca tccaccactc cggatccatg gaccaacgtc
     1921 aaaagtcagg agctagcgga gccgattaca aggatgacga tgacaagaga ggatctggat
     1981 ctgactacaa ggacgatgac gataagcgtt aagataaatg caaaatcctt tcaagcattc
     2041 ccttcttctc tatcactctt ctttcttttt gtcaaaaaat tctctcgcta atttatttgc
     2101 ttttttaatg ttattatttt atgacttttt atagtcactg aaaagtttgc atctgagtga
     2161 agtgaatgct atcaaaatgt gattctgtct gatgtacttt cacaatctct cttcaattcc
     2221 attttgaagt gctttaaacc cgaaaggttg agaaaaatgc gagcgctcaa atatttgtat
     2281 tgtgttcgtt gagtgaccca acaaaaagag gaaactttat tgtgccgcca agaaaaaagt
     2341 ctcacgtacg gtgcgcgcga tgccgtatca caactgagaa aaaagacaaa acttattgaa
     2401 cagtgttcct ttcaaaaaat tattttgtaa aaaatcgagt ttttctccaa aataacgcac
     2461 gcaatcaaaa acaagaaaac taacgtgttc tcctgggcgt ggaactcctt atcaagatta
     2521 ccattattgt ctgtcagaat tgatatttca attatttcat atctagacaa acccgggtgt
     2581 cttgcattta ttagtactgt gagtttacat acaaacgagg cttactcaga taacatagag
     2641 tcacctctaa ttagcaaaaa cccatttcta attagctatt tcgaccagtt ctaaagtcac
     2701 cgtaattaga aatggttttt gtagcttttc taattagaaa tgaccccgag ataagtgaaa
     2761 tatggcattt tctgggtttt ctagtaagaa atgatcccgc acgtagtgat tccgcgttac
     2821 aagccaaatt caaattaaaa aagtatatta cacaattcaa gttacattat tttcaggaaa
     2881 caatggcgaa gagcaaagca gagattcaaa aggaggaaag agacaacaga tatgaacgaa
     2941 gacttcttga aaagagaaag agacatgagg tgagtaacca tgataataaa taatcaagta
     3001 agggctatag atttataaat tctggagtta acagtttcga aaaaaagtag ttttcatatg
     3061 tgcgaaggaa tatcaaaaat aggggaatag cctaaacgag tgagaaaatt aaatgtttgt
     3121 tattttttca aaaatgtctg cagggccgtt atcctttcaa atgcactcta tcaaacatag
     3181 atcatatcca tcatatctta tccagaattt atatcatatc caagattgag agcgtttcaa
     3241 ggaaaatctc aaacaaccat acaattttgt agatcgacaa caaggcctcc acttcttcat
     3301 cgatggcctc tgcagcacct gcaccaccaa gtttgtattt ttcatattaa tataaagtga
     3361 gccacgcata gtcgttttta ggatttttgc cagatcgatg tcgcgcaaac agactgtttt
     3421 tttttctttg aaagccgtgc gtgacatggg ccacggattg taacgccgcg ctggatggat
     3481 aaaaatttat tcagtttttt agcctttaca aaatatggtt cgactacttt ctttattttt
     3541 acatttttat aggtgctcat tgagctgaga tattttgggt ataatcggaa aatcgctgat
     3601 tttggtcatt tttctgcact aaagattaca caaaaagttc agtatttaga ttcattgttg
     3661 aaaattgggg atgatctaca gatacatatc gccgtgttca aaaacacacg tgacgtcgtg
     3721 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     3781 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     3841 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3901 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     3961 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4021 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4081 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4141 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4201 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4261 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4321 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4381 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4441 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4501 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4561 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4621 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4681 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4741 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4801 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4861 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4921 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4981 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5041 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5101 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5161 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5221 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5281 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5341 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5401 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5461 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5521 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5581 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5641 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5701 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5761 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5821 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5881 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5941 tggtcttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6001 ataaccgtat taccgccttt gagtgagctg ataccagcgg ataacaattt cacagggccc
     6061 tgtcggatta tgggctcttc ttttatcctc tactttcttt ctagaaaatg caaatgttga
     6121 gtcagaaatt gctgaatgga gtacaactgc aatgggttta gcttatgttt ctggaacaat
     6181 tactggtgga attgtgattg aaaagtaagg ttatatttca tttcaataaa tgttaccaca
     6241 gtataacaaa aacaagttac attgttcaga gtatgtctga atgtattatt attattgtat
     6301 ttgtgcatca gtactgtagt tgtactgaag aagggtttaa aataatctca acgtttttaa
     6361 gacgagttag tgagaatacg gtcgcagtta ggatgtttgg acatagtttt attagataat
     6421 cgtgaactaa ctcactataa aaacccgcta actcattgaa taagatagtt ttaaacgttc
     6481 aaaatttgaa gacaaaattg tatgaataag tttacagagt tggtcgacgc aagcttctcc
     6541 ttctcttcac atttctcaat aatttagctc taattgcatt cgtattcttc gcaaaaatac
     6601 gtattctgat tgacccgatg aaatacggat gcctagttgc attgatcatc tacggttata
     6661 cttatgggtg aggtttttca aatatatttt gatttaatcg aataatgaaa tttcagaact
     6721 ggagttggtc caatttcgtg gtttatatcc tctgaactgg tacctcaaaa acatcgtagt
     6781 attgctcaat ctgtggcata ttcaatgaat actgtaatgg tggttatcag tacatttaca
     6841 gtattacctc tatattccgt tattggtagt tatgctttcc ttattctata ttcaattcct
     6901 tcatttataa gtatgcttat attatttcgc tacttaccgg aaaccaaagg acgagagatt
     6961 catgatattg tgaatgaact taaaaaaaaa tgaaaacctt tgaaaacgtt catacggccg
     7021 aaattttact tgatcgagcc aattgaactt ttgttaagac ttacagaaaa gtgagagatt
     7081 ttgttacaaa aaagtgagaa aaaagcgaaa atgaacagga aaaagaagaa aaatacaccc
     7141 gaagtatcat ataaaaatta aaaagattgt ttgacctggc ggaacttttt tgcaaaaact
     7201 ctgaatatcc atggcacatg cttcgtgcaa aacaggatcc aaatgaatat caacaagaga
     7261 ttcttgaaga cgacgagcca cttgctcaga gcattgctta tctcgaatga gacccttgtt
     7321 aaaatcattt ttcaaacatt atatgacatt gtcgaaatgt cctcctgatt ccatgatggt
     7381 agcaaactca cttcgtcttt gagccaattt atccaagtcc ttgtaaaaag tattcgaaaa
     7441 ttgttaacgg ataaaatgtt tattataata tcaaaaacaa tttgtcagtt gaccactttt
     7501 tgatatattt gacagaaacc gggatgaatt ggctcaaagt tagggcgccc ttctattaca
     7561 gggtttctga taacaaaccg gttattaact cccaacaagg gatgatttca attcatcatg
     7621 ctcaaattga cccaaattaa gttacatgac aaattcatcg cccttttcaa ctcttcttgg
     7681 ctcatcatct gttattctgt tcctattctg tctgcaccct ataccccttt gcatactctc
     7741 tcgtcattcc tttcttggat agtgtcttct tctccccagc tctgctactt ctatgacttg
     7801 cgcccgctgc ttttccgctc gttcctctcc tctctgacgt cttcttctgc ttcttttctc
     7861 cttccttttt cccttctcaa tcttcctctt tttcccattt ctgtcaatca ttcgaagaag
     7921 aagaagaaga agaccctcat tcatttcatt tttttttctg tcggtgtgtg ctgctcggtt
     7981 aagagtgagc tctctattcc acgtcttctt ctttttcttc ttcgattcga atcaatcact
     8041 ccaccaaaac gcattcgttt ttgggattca ccccgcggtt cgcaataggt ttctttttca
     8101 aattattagc gttataaata gaaaaatggt ggagtttcaa taaaaatgat aatttacaaa
     8161 gtgtattttg attacatgta ctcaaaaagg ttgaaatttt tcataccagt tttccggaaa
     8221 tccatctgat atcattatcg tatttttctt tttaaaaatg ttttcaaaaa aaaacaaaat
     8281 atagctggtt atttggcacc ctctaattac cattttcctg tcacaccaca ctcttttcct
     8341 tcttccactt ctttccgttt tcagccgctt ccaaccaaac cgatatgaaa gccgagcaac
     8401 aacgatcgat tccacccggc tcggcgacct tcccgtcgca ggtgagactc agaaaactag
     8461 agaaaccgct caactaactc ttgatatccg atttcattct tttctctttt ctttttgttg
     8521 aacttttcca tatttccaga tgccacggcc accaccaagc accgaacaag gaatcacaac
     8581 ggaatcggag cttgcgaaga aagctcaaat cactccgaac gacgttttag cacttccggg
     8641 aatcactcaa ggtatcttgc tccctttgga tttcagttta aaacataaat ttaggattct
     8701 tatgctcccc atctgcgaac atctataaca tcgagttcac caagttccaa atccgtgatc
     8761 tggacactga gcaagtgctt ttcgagatcg ccaaaccgga gaacgatcag gagaatgatg
     8821 agtcgccaca ggagtcggca agatacgtgc gttatagatt tgctccaaac tttttgaaac
     8881 tcaaaacggt cggagcaact gtggaattca aagtaggaga catcccaatc catcatttcc
     8941 gaatgatcga acgtcacttc ttcaaagatc gccttctgaa gtgttttgac tttgaattcg
     9001 gattctgtat tccgaattca cgaaacaact gtgaacatat ctatgagttc cctcaactct
     9061 ctcaacaact cagtgagtca ttattctaaa aagtacaatt caaaagacta atctcttttt
     9121 cagtggacga catgatcaac aatccaaacg agactcgttc tgacagcttc tatttcgtcg
     9181 ataacaaact cgtcatgcac aacaaagccg actactcata tgatgcataa atatttaata
     9241 caaaaatgtt ctggataatt attctgtcga atagaaaaaa aactccaaat gtgattaaat
     9301 tccaataatt cctgtctagt ttgttccttc cccttcccct tctcatgttc aatgcattcc
     9361 taagcttttc agttcccccc ttgtttctat attttttcgc tgtcctgtca cactcgctaa
     9421 aaacactaat cacacggaaa tctgttttca ataaaaactc caactttaac tcattttcaa
     9481 tttcaactga aagatttttt cattagagaa tgtctagaac taggcccggg ctacgtaata
     9541 cgactcactt aaggccttga ctagagggta ccagagctca cctaggcagg aacagctatg
     9601 accatgatta cgccaagcta t
//
