LOCUS       sequence-137532-         797 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  TU for combinatorial construction of ?-carotene pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: POT2-TDH3p-ycrtE-ADH1t
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        85..751
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     621..644
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 aagctggagc tccaccgcgg tggcggccgc gaattccccg ggtctagagg tctcggttgg
       61 cagtgactcg gtctctacct ggcttcatta tcaatactgc catttcaaag aatacgtaaa
      121 taattaatag tagtgatttt cctaacttta tttagtcaaa aaattagcct tttaattctg
      181 ctgtaacccg tacatgccca aaataggggg cgggttacac agaatatata acatcgtagg
      241 tgtctgggtg aacagtttat tcctggcatc cactaaatat aatggagccc gctttttaag
      301 ctggcatcca gaaaaaaaaa gaatcccagc accaaaatat tgttttcttc accaaccatc
      361 agttcatagg tccattctct tagcgcaact acagagaaca ggggcacaaa caggcaaaaa
      421 acgggcacaa cctcaatgga gtgatgcaac ctgcctggag taaatgatga cacaaggcaa
      481 ttgacccacg catgtatcta tctcattttc ttacaccttc tattaccttc tgctctctct
      541 gatttggaaa aagctgaaaa aaaaggttga aaccagttcc ctgaaattat tcccctactt
      601 gactaataag tatataaaga cggtaggtat tgattgtaat tctgtaaatc tatttcttaa
      661 acttcttaaa ttctactttt atagttagtc ttttttttag ttttaaaaca ccaagaactt
      721 agtttcgaat aaacacacat aaacaaacaa agatggacta cgctaacatc ttgaccgcta
      781 tcccattgga gttcacc
//
