LOCUS       sequence-137555-        5692 bp    DNA     circular SYN 15-APR-2025
DEFINITION  red opsin promoter from Carolina anole lizard (Anolis carolinensis) 
            gDNA (nucleotides21,797 to 187, corresponding to 
            chr2:88663108–88664997 in anoCar2.0) driving DsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Enright JM, Lawrence KA, Hadzic T, Corbo JC
  TITLE     Transcriptome profiling of developing photoreceptor subtypes reveals
            candidate genes involved in avian photoreceptor diversification.
  JOURNAL   J Comp Neurol. 2015 Mar 1;523(4):649-68. doi: 10.1002/cne.23702. 
            Epub 2014 Dec 1.
  PUBMED    25349106
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.23702";
            journalName: "J Comp Neurol"; date: "2015-03-1- 1"; volume: "523"; 
            issue: "4"; pages: "649-68"
COMMENT     Sequence Label: Ac 1.8kb red opsin DsRed
FEATURES             Location/Qualifiers
     source          1..5692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1948..1957
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1954..2631
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2134..2154)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2546..2569
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2716..2735)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2781..2836
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2835..2854)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3195..3211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3195..3211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3208..3230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3219..3235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3243..3249,3250..3267,3268..3273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3288..3309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3368..3563
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3414..3549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3569..3703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3606..3625)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3660..3679
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3771..3788)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3942..4530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4022..4041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4701..5492,5493..5561))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5324..5343
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5562..5666)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacgata tctctagaga attcatccac ctgtaatgtg attttcctct tctcctactg
       61 ccttttgcct tattggcttt cctggtaaat cagatcttct tatgatatgg ccaaagtaaa
      121 acaacctcag tttagtcatc ccgacttcca gggagagtta aggcttggtt gttttgtttt
      181 tttaaaatta ggacccattt attggtcttc ttagcagtac tttaattaga cctttaagct
      241 tgctaatgat tagctgcttg catcagcctt tttctactaa cttgcatgtc ttttgaatgg
      301 tatactgaag gatgaatcct aaggctgtat gttataccag accaaatagg atgtagatct
      361 tgtgtgctgc gaatagagct tggaaaagta gtttgttgta gtaacaaggg gctctatttt
      421 ggttcggtat cattacagct gcaattcgtt aaaggaaact cttccctttc ttgcttatta
      481 actctcttca caatagtctt ttttgctatt tgtaaaaaaa aacccaaaaa aacccccacc
      541 atcttggaat gttacaagcc catggtgcta aatcctcacc acctcatttg tgccttaaca
      601 gcgcacacga taagatcatg agccaacaat tgaaccatgc caattggaaa aggaaatgtt
      661 acagttccac tgaaaatgga ttggcatcac tcctgttgtg ctaatgagaa ggatgtgaca
      721 ggcatatgac cagctgcaga ggtctttcgc tcccctcttg tctcttagcc agctctgttt
      781 aactgtggga cagaaaaggt tgaagctgct cttcagttac acaagttgaa aggtgacgtt
      841 tttcaaaaac catctatcct gtggcttgga ttgctggcat cagtcatgag gcagtgaact
      901 cttgagtgcc ttgggcccta cttcctttcc ttcttctcca tgccacaaga cacacgggga
      961 tctggaagat ggctctacaa ccacacaaga actgttgtct ttgtggtgtg gttttgccct
     1021 gccctgcaca tgagattctc aggagtgtgc ttttttgtac agtaaccatt tgacatcctg
     1081 tgaataagct ctgggagaca atgcccctgt gttcagcaga catttcctct ctaggaagtg
     1141 ttgatttgtt ttaaaatgtt tttgttgggc tagttttcgt taacaccaca taggagtggc
     1201 tattttctga catattgact tatggtctta tttgccatga gtctgtggtg gactggaaat
     1261 caggcttcgg tgccaaacta ccatctctat agatagcgaa actgggctaa aaggtccctt
     1321 agcctattta gaaaaatggg ccattaggaa attgcaagga agaaccattc gtgagaggga
     1381 ttagctgagc tcttttgact ctctaatcac ccctccgtgc tcatccctca cctgaagtgt
     1441 ccagcaaata caccaagggt gacgcaggac aagcatgagc cattcatact gctgcaacca
     1501 gagagaggga gcaggaaaat gagacaggga gggggccaaa tcacagccca attaagagat
     1561 aaaacgttta agggctttga ggatcttgtt ctccaagtca ggggcttaat ccgcctgggg
     1621 gggtgggtat aaaaatgggc agtgctctgg gccctgtcac tgacgtttgg atgggagaga
     1681 ctgagcggta gagagggaaa gaagagaaga agagagagag gagagaaaag gggggagaga
     1741 gcgaaactag gaagagaagg aaagtgaggg gataggaaag gggcaaaaaa caacaagcgc
     1801 tggtagtagg cgggaaagaa gatggcaggg accgtgacgg aagcctggga tgtggcagta
     1861 tttgctgccc gacggcgcaa tgatgaagac gacaccacaa gggatagcga attcccgacg
     1921 gtaccgcggg cccgggatcc accggtcgcc accatggcct cctccgagaa cgtcatcacc
     1981 gagttcatgc gcttcaaggt gcgcatggag ggcaccgtga acggccacga gttcgagatc
     2041 gagggcgagg gcgagggccg cccctacgag ggccacaaca ccgtgaagct gaaggtgacc
     2101 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc
     2161 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag
     2221 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac cgtgacccag
     2281 gactcctccc tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc
     2341 ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc caccgagcgc
     2401 ctgtaccccc gcgacggcgt gctgaagggc gagacccaca aggccctgaa gctgaaggac
     2461 ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg
     2521 cccggctact actacgtgga cgccaagctg gacatcacct cccacaacga ggactacacc
     2581 atcgtggagc agtacgagcg caccgagggc cgccaccacc tgttcctgta gcggccgcac
     2641 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     2701 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     2761 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     2821 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     2881 tgagtatttg gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt
     2941 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     3001 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     3061 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     3121 tactcccagt catagctgtc cctcttctct tatgaagatc cctcgacctg cagcccaagc
     3181 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3241 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3301 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3361 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3421 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3481 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3541 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa
     3601 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3661 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc
     3721 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3781 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3841 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3901 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3961 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4021 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4081 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     4141 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4201 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4261 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4321 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4381 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4441 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4501 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4561 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4621 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4681 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4741 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4801 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4861 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4921 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4981 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5041 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5101 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5161 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5221 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5281 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5341 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5401 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5461 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5521 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5581 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5641 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tg
//
