LOCUS       sequence-138019-         794 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  entry vector (L4-R1) containing the Notch responsive tp1 element 
            upstream of a basal promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Quillien A, Moore JC, Shin M, Siekmann AF, Smith T, Pan L, Moens CB,
            Parsons MJ, Lawson ND
  TITLE     Distinct Notch signaling outputs pattern the developing arterial 
            system.
  JOURNAL   Development. 2014 Apr;141(7):1544-52. doi: 10.1242/dev.099986. Epub 
            2014 Mar 5.
  PUBMED    24598161
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.099986"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "7"; pages: "1544-52"
COMMENT     Sequence Label: 502 p5E-tp1
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..91
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    536..660
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(732..751)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(733..751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(756..772)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(756..772)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
       61 tttataatgc caactttgta tagaaaagtt gtgcaggtcg actctagagg atcccgactc
      121 atgggaaaat gggcggaagg gcaccgtggg aaaatagtag atcccgactc gtgggaaaat
      181 gggcggaagg gcaccgtggg aaaatagtag atcccgactc gtgggaaaat gggcggaagg
      241 gcaccgtggg aaaatagtag atcccgactc atgggaaaat gggcggaagg gcaccgtggg
      301 aaaatagtag atcccgactc gtgggaaaat gggcggaagg gcaccgtggg aaaatagtag
      361 atcccgactc gtgggaaaat gggcggaagg gcaccgtggg aaaatagtag atccactgta
      421 tgcatcttgg gcataaaagg cagatcactt cagcttctgc ttacacttgc ttttgacaca
      481 actgtgttta cttgcaatcc ctcgaatcgc tagcatcgat tcgaattcaa ggatcacaag
      541 tttgtacaaa aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta
      601 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatgc agtcactatg
      661 aaccaactac ttagatggta ttagtgacct gtagaattcg agctctagag ctgcagggcg
      721 gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
      781 ggcccgtgtc tcaa
//
