LOCUS       sequence-138081-         765 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Express iCre and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  TITLE     plasmid for first generation adenovirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAdx-CMV-iCre-P2A-copGFP
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..698
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
ORIGIN
        1 ttggatccac catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca
       61 tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca
      121 agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct
      181 acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct
      241 acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga
      301 agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg
      361 tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg
      421 acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc
      481 tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg
      541 tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc
      601 ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg
      661 agtaccagca cgccttcaag acccccattg ccttcgccag atcccgcgct cagtcgtcca
      721 attctgccgt ggacggcacc gccggacccg gctccaccgg atctc
//
