LOCUS       sequence-138165-         996 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK404
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(574..591)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(601..618)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             637..659
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    674..698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(698..717)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(699..717)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(801..820)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattccta gcgtgcgttg gggggtacaa tttctggcag
      121 ttcatcaccc ggctgcgggg gtggcagctg gcgagggcgg cggttttcat ccagcgccac
      181 aaaaataaac tcgccttccg tcaccttgga tgtcagatgc gtgtggcggg cccggcgcca
      241 tgtttcaaca tgaatggtga gcgatgtacg ccccgcgcgc aaaatgcggg tgtaaatgct
      301 cacttcatcc cccaccatca cgggttccat aaacacaagg ctgttaatgg caatggtggc
      361 gcaccggcca ttggcccgaa acgccgccgt ggtgccggct gcaagatcca tctgagaaac
      421 aatccacccg ccaaacacgt ctcccgccgg gttggtgtct gtgggcatgg ccaccacgcg
      481 gatggtgggg atatcggttg gggcttggga attttctgag cggggcatgc agtgctgttc
      541 cttctggccg gtgccatagg tttctgacat atggctgccg cgcggcacca ggccgctgct
      601 gtgatgatga tgatgatggc tgctgcccat ggtatatctc cttcttaaag ttaaacaaaa
      661 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt
      721 tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
      781 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
      841 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
      901 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
      961 cctcaaccta ctactgggct gcttcctaat gcagga
//
