LOCUS       sequence-138266-        1018 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  C-terminal tagging with mCherry2 using CRIPSR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK280 (mCherry2-NeoR)
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             64..774
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(209..227)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    892..1013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(929..948)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     983..1002
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttggagctcg gatccggtgc aggcgccgct
       61 agcatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      121 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      181 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      241 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      301 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caattgggag
      361 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
      421 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      481 gtaatgcagt gtcgtaccat gggctgggag gcctccactg agcggatgta ccccgaggac
      541 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      601 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      661 gtcgacatca agttggacat cctttcccac aacgaggact acaccatcgt ggaacagtac
      721 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaatctaga
      781 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
      841 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
      901 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
      961 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcg
//
