LOCUS       sequence-138324-         760 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  csd6, construct:13-330, pET28b(+) N-tag, mutation:Y132A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Kim HS, Im HN, An DR, Yoon JY, Jang JY, Mobashery S, Hesek D, Lee M,
            Yoo J, Cui M, Choi S, Kim C, Lee NK, Kim SJ, Kim JY, Bang G, Han BW,
            Lee BI, Yoon HJ, Suh SW
  TITLE     The Cell Shape-determining Csd6 Protein from Helicobacter pylori 
            Constitutes a New Family of l,d-Carboxypeptidase.
  JOURNAL   J Biol Chem. 2015 Oct 9;290(41):25103-17. doi: 
            10.1074/jbc.M115.658781. Epub 2015 Aug 25.
  PUBMED    26306031
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.658781"; journalName: "J Biol Chem"; date: 
            "2015-10-9- 9"; volume: "290"; issue: "41"; pages: "25103-17"
COMMENT     Sequence Label: csd6_13-330_Y132A/pET28b(+) N-tag
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggtggga ttgaatgcta gtgatcgttt gttagaaatc
      121 atgcgccttt atcaaaaaca aggcttggaa atggtgggtc aaaagttgga ttcttattta
      181 gcggataaat ctttttgggc agaagaactt caaaacaagg acacggattt tggctattat
      241 caaaacaagc agtttttatt tgtggctaat aaatccaagc ccagtttgga gttttatgag
      301 atagaaaata acatgcttaa aaaaatcaac agctctaaag ctcttgtagg ctctaaaaag
      361 ggcgataaga ctttagaggg cgatttggcc acgcctattg gagtgtatcg tatcacgcag
      421 aaattagagc gcttggatca agcgtatggc gttttggctt ttgtaacgaa ttaccctaat
      481 ttgtatgata ccttgaaaaa acgcaccggg catggcattt gggtgcatgg aatgccttta
      541 aatggcgatc ggaatgaatt gaacaccaag ggctgtattg cgattgaaaa cccgctttta
      601 agctcttatg acaaagtgtt aaaaggcgaa aaagcgttcc tcatcaccta tgaagacaag
      661 tttttcccaa gcaccaaaga agaattgagc atgattttaa gctccctttt tcaatggaaa
      721 gaagcctggg ctaggggcga ttttgaacgc tacatgcgtt
//
