LOCUS       sequence-138422-         756 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Nuclear expression of RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Yin QF, Hu SB, Xu YF, Yang L, Carmichael GG, Chen LL
  TITLE     SnoVectors for nuclear expression of RNA.
  JOURNAL   Nucleic Acids Res. 2015 Jan;43(1):e5. doi: 10.1093/nar/gku1050. Epub
            2014 Nov 5.
  PUBMED    25378317
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-01"; volume: "43(1):e5. doi"; pages: " 
            10.1093/nar/gku1050. Epub 2014 Nov 5"
COMMENT     Sequence Label: pZW1-snoVector
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(47..68)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     396..415
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             701..715
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 cttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
       61 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
      121 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      181 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
      241 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
      301 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      361 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      421 agaagatggt gcgctcctgg aagaaagaga ccgcggttaa ttaactgttg gagtaatgtg
      481 ctggacctca gttcgacgag aatgacggta tagagttttc actcattttg ttcagctttc
      541 caaggaatgt atatatggaa gtcatcatcg atccacacac aatcttggta ccgtcgactg
      601 cagaattcga agctttagcc cccggtggac ctcagttcaa ctaagatgat agtacagagt
      661 tcccactcat tttgttcagc tttccaagga atgcatgtat ggaagtcatc attggtccaa
      721 gatctcactc gagacttacc tggacgtagc cttcgg
//
