LOCUS       sequence-138442-        1066 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Produces Acetobacter aceti 1023 Est07.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK378
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(909..920)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcttac agatttagtg tacgtttaag ggctgcgcag
      121 taggcatcca taaacctgcg agaaagttca gcatctggca catacatgtc aaaaacatga
      181 tacgcgccgg gcactaccat aacttctgtt ggcacagccg cagccatcag acgagttgcg
      241 taatccagat cttccttcaa gaaaagatcc attgatccca caccaataaa agtagggggc
      301 agacctgcca gatcagttgc gcggcccgca gcggcataag gtggtgtttg cggcccacct
      361 gctttatccc ccagatacgc ctgccatgca tatttgttgg ccgaccgtgt ccagacaaat
      421 tcaccaaaat tggcgcccgg atcactattt attgcagtgc ggtcatccag catggggtaa
      481 atgagcaact gaaaagccag cttgtattct tttttgtcac gcgccatcag gcttagcgct
      541 gcacttaaac caccgcctgc actttcaccc gcaaccgcaa ccttatcacg cgagatgcca
      601 agcttgctag cattgtcatg cacccatttc agcacggcat aacaatcttc aattgcaccg
      661 ggatatttca cttctggtgc caggcggtaa tctacggaga aaacctgaaa gcccagatca
      721 ctggccagtt tctggcattt gggatcatcc acttcaggat gaccagaaac aaggccacca
      781 ccatgaatat aaaccatggc cggtgcattg ggctgtacat tacgtggcgt ataaacaatc
      841 acgcgtacat ctggggcatt attcaacccc ggcacgtatt cttcccgcgt tacaacatct
      901 ggcagaggcc gcccttcaat aaggcgcaca gatgttagaa aaacctctcg cacttctgga
      961 agtgagagcg atgaaagacc atcaaaagat ggcattttct caagagtggg aagaaattcg
     1021 ggattaacta ctgattttgg catatgtata tctccttctt aaagtt
//
