LOCUS       sequence-138474-       10489 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 10489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD284
FEATURES             Location/Qualifiers
     source          1..10489
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..76
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(91..96,97..114,115..121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     134..156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     153..169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             434..739
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(787..810)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(814..837)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(841..864)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    883..916
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1269..1293
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1294..2319)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1294..2319)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(2322..2346)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4264..4587
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(6404..6433)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    6443..6476
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6494..6514)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(7471..7776)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8042..8059)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8042..8058)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8051..8073)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8267..8286)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8386..8408
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8446..8464)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8532..8636
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8637..8705,8706..9497)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8855..8874)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9668..10256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10157..10176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10410..10427
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgttatcga
      181 ttacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
      241 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg ttcggtaagc
      301 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
      361 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc cgacgagaac
      421 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
      481 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
      541 ggccagtgca cgtctgctgt cagataaagt cccccgtgaa ctttacccgg tggtgcatat
      601 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
      661 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
      721 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgaac
      781 tagtctcttg tcatcgtcat ccttgtaatc acgcttgtcg tcgtcgtcct tgtagtcacg
      841 cttatcgtca tcgtctttat aatcagatcc ggctccctga aaataacttc gtataatgta
      901 tgctatacga agttattaaa aattgcggtc ataaactgaa acgtaacata tgataaggta
      961 ttttgtgtgc ggttttttct atgatgtttc aaaaaaattt atcagaagta aaaaacataa
     1021 gaagtgtaca cagaacattg tgtagatttt gggatagaat tattattaat ggggggaggg
     1081 agcacaattt ttttcgactt tttattacga attaattcta tttttgaatc tacacaattt
     1141 cattgttaga ggtgacttaa aagaagctaa aaaacaaaga aattaagaga agttttttga
     1201 taataacaaa aatagggggt gggagcacag ggagaaagag catgtaggga tgttgaagag
     1261 taattggacc cactttgtac aagaaagctg ggtctattcc tttgccctcg gacgagtgct
     1321 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
     1381 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
     1441 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
     1501 ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg
     1561 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
     1621 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
     1681 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
     1741 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
     1801 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
     1861 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
     1921 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg
     1981 ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg
     2041 gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat
     2101 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
     2161 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
     2221 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
     2281 cttctcgaca gacgtcgcgg tgagttcagg ctttttcatg gagcctgctt ttttgtacaa
     2341 acttgcggca ccgcctgaca tattacctta aaattcaaaa attaatttca gatcagtcat
     2401 aaccaagaaa acaaaataaa cgttaggaca gaaaattcaa attaattaaa aattatgctg
     2461 aataagaaaa ttacatgcaa agccttattt tcctcataga aacagttgaa taacatattt
     2521 tttaattgaa aacatagcaa aattcgtggt aaaatttgtc aaaaccttgg aaaaaatcga
     2581 aaaaagaaca tttcccgatg cattttccta tttctcgttg ccgacaacgt ataaacgttg
     2641 aatactcagt caacgaattt ggctgctggc ttggactctc gcacggaaga gacgcgtgtg
     2701 tagatttacg acgaaagcaa aaatgatcac caaaaacgga acgttgagct ggacggaaat
     2761 agtggtaaag tgacatgatt atagtttgaa gatttctaat ttcacaatta gagcaaatgt
     2821 tgttcggtat ttattttcaa cggtatttat actattttcc acctttttct agaacattcg
     2881 agctgcttgt tgcaaaagga gggcgactca cattcggtac atggaaaagt agtgtacaca
     2941 ataaagagac ccagatacat tttccgtctg cgtctctttg cacccaccgg gagtattttc
     3001 aaacgaatgc atctaggacc ttctagaaca ttctgtaagg ctgcagaatg cgggtatata
     3061 aggaaagcgg gctcagagga agccaacacg ctttgttcta gtgcatctaa aaaacttcga
     3121 aaatatgtca aatcttctca ctgttcatca aaatcttcca gcacttccag ttgacgcgac
     3181 aagtgatgaa gttcgcaaga acctcatgga tatgttccgt gacagacaag ccttctccga
     3241 gcacacctgg aagatgcttc tctcagtgtg ccgttcttgg gctgcctggt gcaagcttaa
     3301 caaccgcaag tggttcccag ccgagccaga agacgttcgt gactacttgc tctacctcca
     3361 agctcgtgga ttggcggtga agacgatcca acaacatctc ggacagctca atatgctcca
     3421 tcgccgatct ggacttccac gcccatcaga ttctaatgcc gtatctctcg taatgagacg
     3481 tatccgcaag gaaaatgttg atgctggaga gagagccaag caagccctcg ccttcgaaag
     3541 gtaagtttaa atattaatgt attcaatcct aagacttgac gaaattttca gaaccgactt
     3601 cgatcaggtc cgttctctta tggaaaattc cgatcgttgc caggacatcc gaaacctcgc
     3661 cttcctcgga attgcttaca acacacttct ccgtatcgcc gaaattgccc gcattcgcgt
     3721 caaggatatt agtcgcacag acggaggaag aatgctcatc cacatcggac gtacaaaaac
     3781 gctggtctcg actgccggag tcgaaaaagc gctctccctt ggagttacca agctcgttga
     3841 gcgctggatt tcggtttccg gagtcgccga tgatccaaac aactacctgt tctgtcgtgt
     3901 tcgcaagaac ggagttgctg ccccgagtgc aacatcacaa ctctcgactc gagctcttga
     3961 aggtaagttt aaacaacata ttagctaaga ataagctgaa aataaatttt caggaatctt
     4021 cgaagctacc caccgtctca tctacggagc caaggatgat tccggacaac gttacttggc
     4081 ttggtctgga cattccgccc gtgtcggagc tgcccgtgac atggctcgcg ctggagtttc
     4141 catcccagag attatgcaag ccggaggttg gaccaacgtc aacattgtga tgaactatat
     4201 tagaaacttg gattctgaaa ctggcgccat ggttcgtctt ctcgaagacg gtgattaaga
     4261 taaatgcaaa atcctttcaa gcattccctt cttctctatc actcttcttt ctttttgtca
     4321 aaaaattctc tcgctaattt atttgctttt ttaatgttat tattttatga ctttttatag
     4381 tcactgaaaa gtttgcatct gagtgaagtg aatgctatca aaatgtgatt ctgtctgatg
     4441 tactttcaca atctctcttc aattccattt tgaagtgctt taaacccgaa aggttgagaa
     4501 aaatgcgagc gctcaaatat ttgtattgtg ttcgttgagt gacccaacaa aaagaggaaa
     4561 ctttattgtg ccgccaagaa aaaagtctca ctgaatctca aatattttat tagaaaacac
     4621 caacaattta gatatttctg tatccacggt tggcgttgac agcctctgat ggtggagact
     4681 tgtctgggca ctggcagtac tcggcatcct ttcctggtct tccgtctggt ccagtctctc
     4741 cctgggttcc gtctggtcct tgacgtcctt gctttccaga tggtccttct ggtccaacgg
     4801 ttcctggctc tcctggtggt ccgtgaagac cgttctgtcc agctggtcct gttggtcctt
     4861 gatctcctct tggtcccttt ggtcctggtc ttccgattgg atgctcggcg tcgcgtccct
     4921 tctctcctgg tggtcctggc tctccgatct ttccgatttg accggatgat ccttgctctc
     4981 ctggatgtcc tggttgtcca tctcttcctg ggtttccatt ttgtcctctt ggtcctggaa
     5041 gtccacgtgg tcctggtctt ccaagagctc ctggtggtcc aactggtcct tgtgggcaag
     5101 tgaagcatcc gacagactcg cgttgtggag cgatatcatc agctccaact cctggaacac
     5161 cgtcaagtcc tggaactccg tcgagtcctg gaactcctgg aactcccttt ggtccggatg
     5221 gtccagctgg gcacttgtta tcagcattgc agttgcaggt tggtccattt ccattggctc
     5281 catttggagt ctttggctgg ttgaagactg gtggaactgc tggtggttga cctggtggag
     5341 cagatgggga tccatcagca tgtggagttg gggtagcgtt ggtctcttca tattgacaac
     5401 ggacacgctt gctagatctt ccgatgacaa ctatgtcctt ccacaatcca ttggtagatt
     5461 gctagaaaat taaaaattat ttaaaattca aaatataatt taactcactc tgaacacatt
     5521 catctcggta tcaagcttct ctctgaatcc atcaacctcc gagtagatgg ttgacatggt
     5581 catgaagcat accataagag tggcgacagt gaccgaagcc gtcacataac acgcaagttt
     5641 tacagacatc tctgaaatta tcaaatttag gatttcaaaa taattcggcc aaagaaatgt
     5701 aactcaccta gtcttcgaga agcagagact tgtcgatttc ctaccggatt cgtctccttt
     5761 tattaggaaa tctttccgca agttttcaag ttttttttgt ttttttttta cattaccgac
     5821 gagacagtgt gcgagacgca gagacgataa aagactggat aagaacggga gataaggagt
     5881 acggatgtag gtgaggaatg tggccgaaag gacagatggg ctttattcgg gaagattggt
     5941 tgggaaatga accagagaag aatgactgaa taaagttgtt gaaacttggt gagaatggaa
     6001 gatagacaat cgagggcagc tagtttcctt cctcctctat atatttttat gttttctttt
     6061 aaaccttcct ccatatttct gaattgaatg ttgttgcata ttgtgtgtcc ttatttcatt
     6121 tccatcatcc ttttgtgtgt ttcccccttc caccaattcc ccccacgtcc taatgaaatg
     6181 aaagcgagaa gataaataaa cttgcacatt gtgttcagat gagaggagcg gaatacgaat
     6241 tgggagacgg aaagagaaaa aaaaatgttt agatttggat tgaattaatt ttttagaaag
     6301 caagaaaaaa aacacaaaga gaataaataa ataataggaa aaaaaacaaa tattcagtat
     6361 ttaaaatttg aaaattcaac gacgttggcg tcgatcatcc ttagaggtcc tcctcggaga
     6421 taagcttttg ctctccctga aaataacttc gtataatgta tgctatacga agttatttta
     6481 aacttacctt tccggattga aagtacagat tctccttgta gagctcgtcc attccgttga
     6541 gcttgtgtcc gagcttggat gggaggtcgc agtaacgggc gacggcgacc tcgtgttgct
     6601 cgacgtaggt ctccttgtcg gcctccttga tacgctcgag acggtggtcg acgtagtaga
     6661 ctcctggcat cttgaggttc ttggctggct tctgaaaatt taaatcagat tagttagtta
     6721 gtaaaatcat gtttaaactt accttggaac ggtaggtggt cttgaagttg cagatgaggt
     6781 gtcctcctcc gacgagcttg agggccatgt cggtacgtcc ctcgagtcct ccgtcggctg
     6841 ggtagagcat ctcggtgttg gcctcccatc cgagggtctt cttttgcatg actggtccgt
     6901 tggatgggaa gttgactcca cggatctgaa aatttaaata tgtatggtta gttagtaccg
     6961 aactgtttaa acttaccttg acgttgtaga tgaggcatcc gtcttggagg gaggtgtctt
     7021 gggtggcggt gaggactcct ccgtcctcgt aggtggtgac acgctcccag gtgaatccct
     7081 ctgggaagga ttgcttgaag aagtctggga ttccttgggt gtggttgatg aaggtacggg
     7141 atccgtacat gaaggaggtg gcgaggatgt cgaaggcgaa tgggagtggt cctccctcga
     7201 cgacctgaaa atttaaataa tcagggttag ttagtatata tatgtttaaa cttaccttga
     7261 tacgcatggt ttgggttccc tcgtatggct ttccctctcc ctcggaggtg cacttgaagt
     7321 ggtggttgtt gacggttccc tccatgtaga gcttcatgtg catgttctcc ttgatgagct
     7381 cctctccctt ggagaccatc gatgctcctg aggctcccga tgctccctag gtcgacctgc
     7441 agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc
     7501 gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac
     7561 cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac
     7621 cgggtaaagt tcacggggga ctttatctga cagcagacgt gcactggcca gggggatcac
     7681 catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg
     7741 gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcgg
     7801 caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg
     7861 ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccgaac
     7921 cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac
     7981 tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtaac tagtgccggc
     8041 gactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     8101 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     8161 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     8221 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     8281 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     8341 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     8401 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     8461 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     8521 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8581 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8641 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8701 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8761 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8821 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8881 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8941 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     9001 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     9061 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     9121 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     9181 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     9241 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9301 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     9361 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9421 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9481 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9541 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9601 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9661 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9721 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9781 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9841 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9901 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9961 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    10021 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    10081 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    10141 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    10201 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10261 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10321 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    10381 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    10441 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatg
//
