LOCUS       sequence-138533-         794 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR
  TITLE     Pathway engineered enzymatic de novo purine nucleotide synthesis.
  JOURNAL   ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p.
  PUBMED    18707057
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2008-08-15- 15"; volume: "3"; issue: "8"; 
            pages: "499-511"
COMMENT     Sequence Label: pET22-HT/plsA
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(80..127)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     123..141
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(194..211)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccaatccgg
       61 atatagttcc tcctttcagc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt
      121 atgctagtta ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag
      181 cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc ttttagccga
      241 ggattttttc cagatcagcg cgaacttcag caaccggctt ggtgccgtca actttcgcgt
      301 atttggtgtt acccgcttcc gcttctttgg agtagtagcc gatcagcggt gcagtcatct
      361 gatggtattc aaccagacgt ttacgcacgg tttcttcctg atcgtcttta cgggtagtca
      421 gttcttcacc ggtaacgtcg tctttgcctt ctactttcgg cggattgaat ttaacgtgat
      481 aaacacgacc agacggcgca tgaacgcggc ggcctacgat acgatcaaca atcagttcgt
      541 ccggtacgtc gaattccaga acgtaatcaa cattgatgcc cgcttctttc atcgcgtctg
      601 cctgcggaat ggtacgcggg aagccgtcca acaggaaacc attacggcag tcttcctgag
      661 caatgcgctc tttaaccagc gcgatcacca gttcgtcggt gaccagtttg ccagcatcca
      721 taatgtcttt tgcttgttta cccagctcgg agccagattt gaccgcagca cgcagcatat
      781 cgccagtgga gatt
//
