LOCUS       sequence-138718-         732 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  receiving vector of standard transcription units(TUs) in YeastFab 
            work, useful for further assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: POT10-RFP
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    193..214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(229..234,235..252,253..259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    267..283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             301..312
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aagctggagc tccaccgcgg tggcggccgc gaattccccg ggtctagagg tctcggttgg
       61 cagtgactcg gtctctgtcg ggctagagac ggcaatacgc aaaccgcctc tagccgcgcg
      121 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      181 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      241 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag
      301 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
      361 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      421 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      481 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      541 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      601 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      661 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      721 gttatgcaga aa
//
