LOCUS       sequence-138768-        1018 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Produces Escherichia coli aminoimidazole ribonucleotide synthetase, 
            N-terminal His6 tag (H6EcPurM).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK633
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..76
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcatcat catcatcatc acggcgaaaa
       61 cctgtatttt cagggcggat ccgtgaccga taaaacctct cttagctaca aagatgccgg
      121 tgttgatatt gacgcgggta atgctctggt tggaagaatc aaaggcgtag tgaagaaaac
      181 gcgtcgtccg gaagtgatgg gcggtctggg cggcttcggt gcgctgtgtg cattgccgca
      241 aaaatatcgt gaacccgtgc tggtttccgg cactgacggc gtaggtacca agctgcgtct
      301 ggcaatggac ttaaaacgtc acgacaccat tggtattgat ctggtcgcca tgtgcgttaa
      361 tgacctggtg gtgcaaggtg cagaaccgct gtttttcctc gactattacg caaccggaaa
      421 actggatgtt gataccgctt cagcggtgat cagcggcatc gcggaaggtt gtctgcaatc
      481 gggctgttca ctggtgggtg gcgaaacggc agaaatgccg gggatgtatc acggtgaaga
      541 ttacgatgtc gcgggtttct gcgtgggcgt ggtagaaaaa tcagaaatca tcgacggctc
      601 taaagtcagc gacggcgatg ttctgattgc actcggttcc agcggtccgc actcgaacgg
      661 ctattcgctg gtgcgcaaaa ttcttgaagt cagcggttgt gatccgcaaa ccaccgaact
      721 tgatggtaag ccattagccg atcatctgct ggcaccgacc cgcatttacg tgaagtcagt
      781 gctggagttg attgaaaagg tcgatgtgca tgccattgcg cacctgaccg gcggcggctt
      841 ctgggaaaac attccacgcg tattgccaga taatactcag gcagtgattg atgaatcttc
      901 ctggcagtgg ccggaagtgt tcaactggct gcaaacggca ggtaacgttg agcgccatga
      961 aatgtatcgc accttcaact gcggcgtcgg gatgattatt gccctgcctg ctccggaa
//
