LOCUS       sequence-138797-         756 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  bacterial expression of PH domain for phosphoinositide binding 
            assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray 
            D, Emr SD, Lemmon MA
  TITLE     Genome-wide analysis of membrane targeting by S. cerevisiae 
            pleckstrin homology domains.
  JOURNAL   Mol Cell. 2004 Mar 12;13(5):677-88.
  PUBMED    15023338
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2004-03-12"; volume: "13(5)"; pages: "677-88"
COMMENT     Sequence Label: pGEX2TK-Skm1-PH
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(444..461)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(488..510)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 atcgtcagtc agtcacgatg aattcatttg tgtgtaaaat ttgttggtga tgaaacacca
       61 ctaaggagag gacatttggc aaaaatggca tcaagccagc catgcaaatc ccgttcggtt
      121 cttgtggaaa tgaatatcga tcttttggaa tttgattcat aaaagtagga gtttatgggg
      181 gatatgttct ctttttgatc tgttgcccga agaatctcaa aacaattttg tttcaattgt
      241 attctgctaa catttgttat actagtcaaa ggcacagata agactggttc ctcattgcac
      301 ttatcacttt tgtaaaatgc taaatacgaa tcattcagta ccaagtatct cttttgccat
      361 aagaacgaaa acaatccatc aactttataa gatatccatc cttccttttt tacgcccttc
      421 atggatccaa cagatgcacg acgagatcca cgcggaacca gatccgattt tggaggatgg
      481 tcgccaccac caaacgtggc ttgccagccc tgcaaaggcc atgctatata cttgctggat
      541 ttcaagtact tatcaatttg tgggatagct tcaatacgtt ttttaaaaca aactaatttt
      601 gggaacgcat ccaggcacat tgggtccatg tataaaacaa catcaagagc gtcatacaac
      661 atgaagtcag gatgggttac atgatcacca tttaaatatg ttttatgaca taaacgatct
      721 tcgaacattt tcagcatttc aggtagcttg ctaaga
//
