LOCUS       sequence-139102-         769 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant 3H5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-3H5
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   388..423
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   454..489
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(490..621)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 atgcgtccgg cgtagaggat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata
       61 gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct
      121 ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag
      181 tgactggcga tgctgtcgga atggacgatc acactactct tcttttgcct attataacat
      241 gaaaacgctt taacagaaag aataggaagg tatccgactg ctggtattaa ccctctttct
      301 caagttatca tcggcaatgt tgaatggagt ccattcaaaa cagcatagct ctaaaacatg
      361 gaactcaaca agtctcagtg tgctgaagtt ttgggaccat tcaaaacagc atagctctaa
      421 aacggatagt gagtcgtatt aatttcgcgg gatgttttgg gaccattcaa aacagcatag
      481 ctctaaaacc tcgtagacta tttttgtcta aaaaatttcg taatcgcact atttgtctca
      541 gctagacttc agtcttgaaa agcccctgta ttactgcatt tattaagagt attataccat
      601 atttttagtt attaagaaat aatcttcatc taaaatatac ttcagtcacc tcctagctga
      661 ctcaaatcaa tgcgtgtttc ataaagacca gtgatggatt gatggataag agtggcatct
      721 aaaacttctt ttgtagacgt atatcgttta cgatcaattg ttgtatcaa
//
