LOCUS       sequence-139139-       10033 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Patterson-Fortin J, Shao G, Bretscher H, Messick TE, Greenberg RA
  TITLE     Differential regulation of JAMM domain deubiquitinating enzyme 
            activity within the RAP80 complex.
  JOURNAL   J Biol Chem. 2010 Oct 1. 285(40):30971-81.
  PUBMED    20656689
REFERENCE   2  (bases 1 to 10033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Oct 1. 285(40):30971-81."
COMMENT     Sequence Label: pOZ-N-FH RAP80
FEATURES             Location/Qualifiers
     source          1..10033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             747..1216
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1281..1638
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1703..2119
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2049..2071
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2087..2103
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    2129..2503
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2441..2457
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2530..2553
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2566..2592
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2566..2592
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             4038..4046
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    4833..5382
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4977..4994)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5204..5223
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             6473..6942
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7638..7655)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7638..7654)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7647..7669)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7863..7882)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7982..8004
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8042..8060)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8128..8232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8233..8301,8302..9093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8451..8470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9264..9852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9753..9772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10006..10023
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttt
      241 gctcttagga gtttcctaat acatcccaaa ctcaaatata taaagcattt gacttgttct
      301 atgccctagg gggcgggggg aagctaagcc agcttttttt aacatttaaa atgttaattc
      361 cattttaaat gcacagatgt ttttatttca taagggtttc aatgtgcatg aatgctgcaa
      421 tattcctgtt accaaagcta gtataaataa aaatagataa acgtggaaat tacttagagt
      481 ttctgtcatt aacgtttcct tcctcagttg acaacataaa tgcgctgctg agaagccagt
      541 ttgcatctgt caggatcaat ttcccattat gccagtcata ttaattacta gtcaattagt
      601 tgatttttat ttttgacata tacatgtgaa agaccccacc tgtaggtttg gcaagctagc
      661 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag aaaagttcag
      721 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
      781 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
      841 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
      901 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
      961 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     1021 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag
     1081 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     1141 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     1201 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca
     1261 ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc cgattgtcta
     1321 gtgtctatga ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc
     1381 tggcggaccc gtggtggaac tgacgagttc ggaacacccg gccgcaaccc tgggagacgt
     1441 cccagggact tcgggggccg tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt
     1501 tggactcttt ggtgcacccc ccttagagga gggatatgtg gttctggtag gagacgagaa
     1561 cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc
     1621 cgcgcgtctt gtctgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta
     1681 tttgtctgaa aatatgggcc cgggccagac tgttaccact cccttaagtt tgaccttagg
     1741 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1801 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1861 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     1921 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     1981 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     2041 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     2101 cactccttct ctaggcgccc ccatatggcc atatgagatc ctatatgggg cacccccgcc
     2161 ccttgtaaac ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc
     2221 tcacttacag gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta
     2281 ccaagaacaa ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg
     2341 ggtccgccga caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct
     2401 gctgaccacc cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca
     2461 cgtgaaggct gccgaccccg ggggtggacc atcctctaga ctgccagatc ttccgctcga
     2521 tgaaccatgg actacaagga cgacgatgac aagctcgatg gaggataccc ctacgacgtg
     2581 cccgactacg ccggaggact cgagccacgg agaaagaaaa aagttaaaga agtctccgaa
     2641 tctcggaacc tggagaagaa ggatgtggaa actaccagtt ctgtcagtgt gaagaggaag
     2701 cgtagacttg aggatgcatt cgttgtgata tccgatagtg atggagagga accaaaggag
     2761 gaaaatgggt tgcagaaaac gaagacaaaa cagtcgaata gagcaaagtg tttggccaaa
     2821 agaaaaatcg cacagatgac agaagaagaa cagtttgctc tggctctcaa aatgagtgag
     2881 caggaagcta gggaggtgaa cagccaggag gaggaagaag aggagctctt gaggaaagcc
     2941 attgctgaaa gcctgaatag ttgccggcct tctgatgctt ccgctaccag atctcgacct
     3001 ctggccactg gaccgtcttc ccagtcccat caagagaaaa ccacagactc tgggctcact
     3061 gaaggcatat ggcagctggt acctccatca ctgtttaaag gctcacatat cagtcaggga
     3121 aacggggctg aggaaagaga ggagccttgg gaccacactg aaaaaactga agaggagccg
     3181 gtctctggca gctcaggaag ctgggaccag tcaagccagc cagtgtttga gaatgtgaac
     3241 gttaaatctt ttgacagatg tactggccac tcggctgagc acacacagtg tgggaagcca
     3301 caggaaagta ctgggagggg ttctgctttt ctcaaagctg tccagggtag cggggacaca
     3361 tctaggcact gtctacctac cctagcagat gccaaaggtc tccaggacac tgggggcact
     3421 gtgaactatt tctggggtat tccattctgc cctgatggag tagaccctaa ccagtatacc
     3481 aaggtcattc tctgccagtt ggaggtttat caaaagagcc tgaaaatggc tcagaggcag
     3541 ctccttaata aaaaaggttt tggggaacca gtgttgccta gacctccttc tctgatccag
     3601 aatgaatgtg gccaaggaga gcaggctagt gagaaaaatg aatgcatctc agaagatatg
     3661 ggagatgaag acaaagagga gaggcaggag tctagggcat ctgactggca ctcaaaaacc
     3721 aaggatttcc aggaaagctc aattaaaagc ttgaaagaga aacttttgtt ggaggaagaa
     3781 ccaacaacca gtcatggtca gtcttcccaa gggattgttg aagaaacttc tgaagaggga
     3841 aactctgtac ctgcttcaca aagtgttgct gctttgacca gtaagagaag cttagtcctt
     3901 atgccagaga gttctgcaga agaaatcact gtttgtcctg agacccagct aagttcctct
     3961 gaaacttttg accttgaaag agaagtctct ccaggtagca gagatatctt ggatggagtc
     4021 agaataataa tggcagataa ggaggttggt aacaaggaag atgctgagaa ggaagtagct
     4081 atttctacct tctcatccag taaccaggta tcctgcccgc tatgtgacca atgctttcca
     4141 cccacaaaga ttgaacgaca tgccatgtac tgcaatggtc tgatggagga agatacagta
     4201 ttgactcgga gacaaaaaga ggccaagacc aagagtgaca gtgggacagc tgcccagact
     4261 tctctagaca ttgacaagaa tgagaagtgt tacctctgta aatccctggt cccatttaga
     4321 gagtatcagt gtcatgtgga ctcctgtctc cagcttgcaa aggctgacca aggagatgga
     4381 cctgaaggga gtggaagagc atgttcaact gtggagggga agtggcagca gaggctgaag
     4441 aacccaaagg aaaaaggcca cagtgaaggc cgactcctta gtttcttgga acagtctgag
     4501 cacaagactt cagatgcaga catcaagtct tcagaaacag gagccttcag ggtgccttca
     4561 ccagggatgg aagaggcagg ctgcagcaga gagatgcaga gttctttcac acgtcgtgac
     4621 ttaaatgaat ctcccgtcaa gtcttttgtt tccatttcag aagccacaga ttgcttagtg
     4681 gactttaaaa agcaagttac tgtccagcca ggtagtcgga cacggaccaa agctggcaga
     4741 ggaagaagga gaaaattctg agcggccgca tcgagagatc cgggagacac aacggtttcc
     4801 ctctagcggg atcaattccg cccccccccc taacgttact ggccgaagcc gcttggaata
     4861 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     4921 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     4981 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     5041 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     5101 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     5161 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     5221 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     5281 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     5341 gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatggat tcatacctgc
     5401 tgatgtgggg actgctcacg ttcatcatgg tgcctggctg ccaggcagag ctctgtgacg
     5461 atgacccgcc agagatccca cacgccacat tcaaagccat ggcctacaag gaaggaacca
     5521 tgttgaactg tgaatgcaag agaggtttcc gcagaataaa aagcgggtca ctctatatgc
     5581 tctgtacagg aaactctagc cactcgtcct gggacaacca atgtcaatgc acaagctctg
     5641 ccactcggaa cacaacgaaa caagtgacac ctcaacctga agaacagaaa gaaaggaaaa
     5701 ccacagaaat gcaaagtcca atgcagccag tggaccaagc gagccttcca ggtcactgca
     5761 gggaacctcc accatgggaa aatgaagcca cagagagaat ttatcatttc gtggtggggc
     5821 agatggttta ttatcagtgc gtccagggat acagggctct acacagaggt cctgctgaga
     5881 gcgtctgcaa aatgacccac gggaagacaa ggtggaccca gccccagctc atatgcacag
     5941 gtgaaatgga gaccagtcag tttccaggtg aagagaagcc tcaggcaagc cccgaaggcc
     6001 gtcctgagag tgagacttcc tgcctcgtca caacaacaga ttttcaaata cagacagaaa
     6061 tggctgcaac catggagacg tccatattta caacagagta ccaggtagca gtggccggct
     6121 gtgttttcct gctgatcagc gtcctcctcc tgagtgggct cacctggcag cggagacaga
     6181 ggaagagtag aagaacaatc tagaggatcc ggattagtcc aatttgttaa agacaggata
     6241 tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac
     6301 gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     6361 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     6421 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat
     6481 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     6541 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     6601 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
     6661 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     6721 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     6781 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
     6841 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     6901 tctcctctga gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa
     6961 attaatttgg ttttttttct taagtattta cattaaatgg ccatagtact taaagttaca
     7021 ttggcttcct tgaaataaac atggagtatt cagaatgtgt cataaatatt tctaatttta
     7081 agatagtatc tccattggct ttctactttt tcttttattt ttttttgtcc tctgtcttcc
     7141 atttgttgtt gttgttgttt gtttgtttgt ttgttggttg gttggttaat ttttttttaa
     7201 agatcctaca ctatagttca agctagacta ttagctactc tgtaacccag ggtgaccttg
     7261 aagtcatggg tagcctgctg ttttagcctt cccacatcta agattacagg tatgagctat
     7321 catttttggt atattgattg attgattgat tgatgtgtgt gtgtgtgatt gtgtttgtgt
     7381 gtgtgattgt gtatatgtgt gtatggttgt gtgtgattgt gtgtatgtat gtttgtgtgt
     7441 gattgtgtgt gtgtgattgt gcatgtgtgt gtgtgtgatt gtgtttatgt gtatgattgt
     7501 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgttgtgta tatatattta
     7561 tggtagtgag aggcaacgct ccggctcagg tgtcaggttg gtttttgaga cagagtcttt
     7621 cacttagctt ggaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     7681 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     7741 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     7801 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     7861 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     7921 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     7981 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     8041 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     8101 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8161 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8221 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8281 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8341 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8401 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8461 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8521 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8581 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8641 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8701 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8761 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8821 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8881 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8941 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9001 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9061 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9121 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9181 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9481 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9541 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9601 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9661 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9721 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9781 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9841 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9901 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9961 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10021 gaggaagcgg aag
//
