LOCUS       sequence-139331-         770 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Reporter plasmid encoding mCherry, codon optimized for E. coli, 
            transcriptionally driven by orthogonal T7-lac promoter variant 4A6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-4A6-mCherry
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agggtacctt atttgtacag ttcatccata ccgccggtag agtggcggcc ttctgcacgt
      121 tcgtactgtt caacgatcgt atagtcttcg ttatggctgg tgatatccag cttgatgttc
      181 acgttgtatg cacccggcag ttggaccggc tttttagcct tgtacgtggt tttcacttct
      241 gcgtcatagt gaccgccatc cttcagtttc aggcgctgct tgatttcgcc tttcagggca
      301 ccgtcttccg ggtacatacg ttcggaactc gcttcccagc ccatcgtctt tttctgcata
      361 accggaccat cgctcgggaa attggtgccg cgcagcttca ctttataaat aaattcaccg
      421 tcttgcagag agctatcctg cgtgacggta accacaccgc catcttcgaa gttcataaca
      481 cgttcccatt tgaagccttc cggaaaggac agtttcagat agtccgggat atcggccgga
      541 tgcttaacgt acgctttcga accatacatg aattgcggtg acagaatatc ccaagcaaac
      601 ggcagcggac cgcctttggt gaccttcagt tttgccgtct gggtgccttc atacggacgg
      661 ccttcacctt cgccttcaat ttcaaattcg tgaccattga cgctgccttc catgtggacc
      721 ttgaaacgca taaattcctt gataatcgcc atgttgtctt cttcgccttt
//
