LOCUS       sequence-139354-        1097 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, 
            Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole 
            ribonucleotide synthetase (EcPurK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Kappock
  TITLE     Single Trp variants of Acetobacter aceti PurE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK501
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgc atgcctgcag ttaaccgaac ttactctgcg cccaaatcac gccgctggca
      121 tattccggcg gcagcagcgg gattaaggct tccagcgtcg cagtcagacg cgatgtgtcg
      181 ctgtcggtca aattcagatg ccccacttta cgccccggac ggacttcttt gtcgtaccag
      241 tgcagatgca ccagcggcag tttcagccag tcataattca catcgctacc aatcagattg
      301 atcatcaccg acggattatt caccactggt tgcggtaacg gcagatcggt aatcgcccgc
      361 agatgcagct caaactggct gatgctggca ccgttttgtg tccagtgacc gctgttatgc
      421 acacgcggtg ccagttcgtt gatcaacaga ccttgcgggg tgacaaaaca ctccatcgcc
      481 atcacgccca catagcccag ctcctgcata atcgccgaca gcatctcttc ggctcgcgcc
      541 tgctgctgtg cgttggcctg cggaaaagcg acgctggtgc gcaaaatacc gtcctgatgc
      601 aggttatgcg tcagcggata aaacacggtg ctgccatcaa agccgcgcgc gccaaccagc
      661 gacacttcac cagagaagtt aatgccctgc tcgacaatac attcgccgta acactctgcc
      721 ggtaactgtt cggtttcatt tgcgcgtaaa cgccattgac cgcgaccgtc ataaccacca
      781 gtgcgacgct taacaatcgc cagctcacct aaacgatcaa acaccgcagg ccactcgctg
      841 cgttcggcaa gtaactgcca cggtgcagtc ggcaggtgga gcttatcgaa aagctgcttc
      901 tgagtcagac ggtcagcaat aatcgggaac acatcgcggt tcacaaaggc cggatggcgc
      961 gccagctcgc gggttaatgc ggtttccggc cagcgttcta tctcagcggt aatcacgctt
     1021 tgttgaaaag gcaccgccgc cggttcagcg tccagcccga ctggccagac agcaatgcct
     1081 aacggttcgc ctgcctg
//
