LOCUS       sequence-139368-        1007 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Produces Acetobacter aceti 1023 small hypothetical protein adjacent 
            to purEK genes (AaOrfX).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK521
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(9..31)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     131..150
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(390..406)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(390..406)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(403..425)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    414..430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(438..444,445..462,463..468))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    483..504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(621..638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(872..891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
       61 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
      121 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgct gcgtgatcgc
      181 gacctgctgc tggcgcaatt gcatgaactg cgtagcgaac accgcgatct ggacaccgtg
      241 atcaaccgta tggcccatga aaccacgttt atcgatcagc tgtatctgca acgtctgaaa
      301 aaacgcaaac tgctgcttaa ggatcaaatt acgaaagtgg aaagtctact gattccggat
      361 gacatcgcgt gaagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
      421 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      481 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      541 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      601 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      661 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      961 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatct
//
