LOCUS       sequence-139597-         988 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  SapTrap TEV-Flagtag N-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS383
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(105..128)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(141..161)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(197..213)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(197..213)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(250..270)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(250..268)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(289..305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(289..305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(407..426)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(485..504)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatgtgct
       61 cttcccgtgc ttccgccggt acctccactg ccaccgctag tactcttgtc gtcgtcgtcc
      121 ttgtagtcag tggttggctc gctctgaaag tacagattct cacccgaaga gcagatcgaa
      181 ttcctgcagc ccgggggatc cactagttct agagcggccg ccaccgcggt ggagctccag
      241 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
      301 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
      361 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
      421 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
      481 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
      541 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
      601 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
      661 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
      721 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
      781 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
      841 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
      901 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
      961 aaacttttgc cattctcacc ggattcag
//
