LOCUS       sequence-139715-       11371 bp    DNA     circular SYN 14-APR-2025
DEFINITION  expression of improved FLP recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 11371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF295
FEATURES             Location/Qualifiers
     source          1..11371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2072..2092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2096..3367
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MSQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPLSKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     polyA_signal    3489..3570
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3526..3545)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3580..3599
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     gene            3612..7740
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(3621..3639)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4091..4162,4557..4830,4905..5559,5621..5936,6157..6288,
                     6359..6973)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(7751..8305,8306..8336)
                     /label=P element 5' end
     rep_origin      complement(8571..9159)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8651..8670)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9330..10121,10122..10190))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9953..9972
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10191..10295)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10363..10381
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10419..10441)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10541..10560
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    10581..10614
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     misc_feature    join(11139..11169,11170..11371)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 aagcttgggc tgcaggtcga ctctagctag aggatgtcgc cggaacgcag cgacagggat
       61 tccaatgtgt ccgtatcctt caggcttttg cccttcagtt ccaggcgcag cgactggcga
      121 ttcgcgtgtg gggtctgctt cagggtcttg tggattaggg cgcgcagatc gccgatgggc
      181 gtggcgccgg agggcacctt caccttgccg tacggcttgc tgttcttcgc gttcaaaatc
      241 tccagctcca ttttgctttc ggtgcgcttg caatcagtac tgtccaaaat cgaaaatcgc
      301 cgaaccgtag tgtgaccgtg cggggctctg cgaaaataaa cttttttagg tatatggcca
      361 cacacgggga aagcacagtg gattatatgt attaatatat tatgcaggtt ttcattactt
      421 atccagatgt aagcccactt aaagcgattt aacaattatt tgccgaaaga gtataaacaa
      481 atttcacata aaaatggatt aagaaaagct tgtgtaagat tatgcgcagc gttgccagat
      541 agctccattt aaaacacttc aaaaacaata agtttagaaa atatatacat aaatagcagt
      601 cgttgccgca acgctcaaca catcacactt ttaaaacacc ctttacctac acagaattac
      661 tttttaaatt tccagtcaag ctgcgagttt caaaattata gccggtagag aagacagtgc
      721 tatttcaaaa gcaaactaac aagggtctta aatttcaaaa caccaatcct aacaagcctt
      781 ggacttttgt aagtttagat caaaggtggc attgcattca atgtcatggt aagaagtagg
      841 tcgtctaggt agaaatcctc attcagccgg tcaagtcagt acgagaaagg tctcaatttg
      901 aaattgtctt aaaaatattt tattgttttg tactgtggtg agtttaaacg aaaaacacaa
      961 aaaaaaagtg atacacagaa atcataaaaa attttaatac aaggtattcg tacgtatcaa
     1021 aaacatttcg gcacaatttt ttttctctgt actaaagtgt tacgaacact acggtatttt
     1081 ttagtgattt tcaacggaca ccgaaggtat ataaacagcg ttcgcgaacg gtcgccttca
     1141 aaaccaattg acatttgcag cagcaagtac aagtagaaag taaagcgcaa tcagcgaaaa
     1201 atttatactt aattgttggt gattaaagta caattaaaag aacattctcg aaagtcacaa
     1261 gaaacgtaag tttttaactc gctgttacca attagtaata agagcaacaa gacgttgagt
     1321 aatttcaaga aaaactgcat ttcaaggtct ttgttcggcc attttttttt tattcaacgc
     1381 tctacgtaat tacaaaataa gaaattggca gccacgcatc ttgttttccc aatgaattgg
     1441 catcaaaacg caaacaaatc tataaataaa acttgcgtgt tgattttcgc caagatttat
     1501 tggcaaattg tgaaattcgc agtgacgcat ttgaaaattc gagaaatcac gaacgcactc
     1561 gagcatttgt gtgcatgtta ttagttagtt gtttagttaa ttgaagtatt taccaacgaa
     1621 atccacttat ttttagctga aatagagtag gttgcttaaa caaagccacg tctgaaaatt
     1681 tcttattgct tgtagttgtg acgtcaccat atacacacaa aataatgtgt atgcatgcat
     1741 ttcagctgtg tatatataca tgcacacact cgcaacacga aaacgatgac gaagcaacgg
     1801 aacaaaggtt tctcaactac cctttgttcc ctgtttcttc gctttccttt gttccaatat
     1861 tcgtagaggg ttaatagggg tttctcaaca aagttggcgt cgataaataa gtttcccatt
     1921 tttattcccc agccagaagt tagtttcaat agttttgtaa tttcaacgaa actcatttga
     1981 tttcgtacta attttccaca tctctatttt ctgcccgcag aataatccaa actgcaggtc
     2041 gactctagct agaggatcct ccaaaatggt gccaaagaaa aagcgtaagg tctcgatgtc
     2101 acaatttggt atattatgta aaacaccacc taaggtgctt gttcgtcagt ttgtggaaag
     2161 gtttgaaaga ccttcaggtg agaaaatagc attatgtgct gctgaactaa cctatttatg
     2221 ttggatgatt acacataacg gaacagcaat caagagagcc acattcatga gctataatac
     2281 tatcataagc aattcgctga gtttcgatat tgtcaataaa tcactccagt ttaaatacaa
     2341 gacgcaaaaa gcaacaattc tggaagcctc attaaagaaa ttgattcctg cttgggaatt
     2401 tacaattatt ccttactatg gacaaaaaca tcaatctgat atcactgata ttgtaagtag
     2461 tttgcaatta cagttcgaat catcggaaga agcagataag ggaaatagcc acagtaaaaa
     2521 aatgcttaaa gcacttctaa gtgagggtga aagcatctgg gagatcactg agaaaatact
     2581 aaattcgttt gagtatactt cgagatttac aaaaacaaaa actttatacc aattcctctt
     2641 cctagctact ttcatcaatt gtggaagatt cagcgatatt aagaacgttg atccgaaatc
     2701 atttaaatta gtccaaaata agtatctggg agtaataatc cagtgtttag tgacagagac
     2761 aaagacaagc gttagtaggc acatatactt ctttagcgca aggggtagga tcgatccact
     2821 tgtatatttg gatgaatttt tgaggaattc tgaaccagtc ctaaaacgag taaataggac
     2881 cggcaattct tcaagcaata aacaggaata ccaattatta aaagataact tagtcagatc
     2941 gtacaataaa gctttgaaga aaaatgcgcc ttattcaatc tttgctataa aaaatggccc
     3001 aaaatcccac attggaagac atttgatgac ctcatttctt tcaatgaagg gcctaacgga
     3061 gttgactaat gttgtgggaa attggagcga taagcgtgct tctgccgtgg ccaggacaac
     3121 gtatactcat cagataacag caatacctga tcactacttc gcactagttt ctcggtacta
     3181 tgcatatgat ccattatcaa aggaaatgat agcattgaag gatgagacta atccaattga
     3241 ggagtggcag catatagaac agctaaaggg tagtgctgaa ggaagcatac gataccccgc
     3301 atggaatggg ataatatcac aggaggtact agactacctt tcatcctaca taaatagacg
     3361 catataagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     3421 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     3481 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3541 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3601 atgtatgaat tctagtatgt atgtaagtta ataaaaccca tttttgcgga aagtagataa
     3661 aaaaaacatt tttttttttt actgcactgg atatcattga acttatctga tcagttttaa
     3721 atttacttcg atccaagggt atttgatgta ccaggttctt tcgattacct ctcactcaaa
     3781 atgacattcc actcaaagtc agcgctgttt gcctccttct ctgtccacag aaatatcgcc
     3841 gtctctttcg ccgctgcgtc cgctatctct ttcgccaccg tttgtagcgt tacgtagcgt
     3901 caatgtccgc cttcagttgc attttgtcag cggtttcgtg acgaagctcc aagcggttta
     3961 cgccatcaat taaacacaaa gtgctgtgcc aaaactcctc tcgcttctta tttttgtttg
     4021 ttttttgagt gattggggtg gtgattggtt ttgggtgggt aagcagggga aagtgtgaaa
     4081 aatcccggca atgggccaag aggatcagga gctattaatt cgcggaggca gcaaacaccc
     4141 atctgccgag catctgaaca atgtgagtag tacatgtgca tacatcttaa gttcacttga
     4201 tctataggaa ctgcgattgc aacatcaaat tgtctgcggc gtgagaactg cgacccacaa
     4261 aaatcccaaa ccgcaattgc acaaacaaat agtgacacga aacagattat tctggtagct
     4321 gttctcgcta tataagacaa tttttgagat catatcatga tcaagacatc taaaggcatt
     4381 cattttcgac tatattcttt tttacaaaaa atataacaac cagatatttt aagctgatcc
     4441 tagatgcaca aaaaataaat aaaagtataa acctacttcg taggatactt cgttttgttc
     4501 ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca ttgcagggtg
     4561 acagcggagc ggcttcgcag agctgcatta accagggctt cgggcaggcc aaaaactacg
     4621 gcacgctcct gccacccagt ccgccggagg actccggttc agggagcggc caactagccg
     4681 agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat cagccgggct
     4741 ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag cgacacatac
     4801 cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc ggctgatctg
     4861 tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg gcgtggccta
     4921 tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa
     4981 tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga tgcgactgct
     5041 caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg tccagcagga
     5101 tgacctcttt atcggctccc taacggccag ggaacacctg attttccagg ccatggtgcg
     5161 gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg tgatccagga
     5221 gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg tgaaaggtct
     5281 gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gcactaaccg atccgccgct
     5341 tctgatctgc gatgagccca cctccggact ggactcattt accgcccaca gcgtcgtcca
     5401 ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc atcagccgtc
     5461 ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca gggtagcttt
     5521 cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat gtgtttatta
     5581 agggtatcta gcattacatt acatctcaac tcctatccag cgtgggtgcc cagtgtccta
     5641 ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc ggacgggaga
     5701 tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgctattagc aaagtagccc
     5761 gggatatgga gcagttgttg gccaccaaaa atttggagaa gccactggag cagccggaga
     5821 atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg tggcgatcct
     5881 ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag acaacggtga
     5941 gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa attcgctaga
     6001 ttttagttag aattgcctga ttccacaccc ttcttagttt ttttcaatga gatgtatagt
     6061 ttatagtttt gcagaaaata aataaatttc atttaactcg cgaacatgtt gaagatatga
     6121 atattaatga gatgcgagta acattttaat ttgcagatgg ttgccatctt gattggcctc
     6181 atctttttgg gccaacaact cacgcaagtg ggcgtgatga atatcaacgg agccatcttc
     6241 ctcttcctga ccaacatgac ctttcaaaac gtctttgcca cgataaatgt aagtcttgtt
     6301 tagaatacat ttgcatatta ataatttact aactttctaa tgaatcgatt cgatttaggt
     6361 gttcacctca gagctgccag tttttatgag ggaggcccga agtcgacttt atcgctgtga
     6421 cacatacttt ctgggcaaaa cgattgccga attaccgctt tttctcacag tgccactggt
     6481 cttcacggcg attgcctatc cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa
     6541 ctgcctggcg ctggtcactc tggtggccaa tgtgtcaacg tccttcggat atctaatatc
     6601 ctgcgccagc tcctcgacct cgatggcgct gtctgtgggt ccgccggtta tcataccatt
     6661 cctgctcttt ggcggcttct tcttgaactc gggctcggtg ccagtatacc tcaaatggtt
     6721 gtcgtacctc tcatggttcc gttacgccaa cgagggtctg ctgattaacc aatgggcgga
     6781 cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa
     6841 ggtcatcctg gagacgctta acttctccgc cgccgatctg ccgctggact acgtgggtct
     6901 ggccattctc atcgtgagct tccgggtgct cgcatatctg gctctaagac ttcgggcccg
     6961 acgcaaggag tagccgacat atatccgaaa taactgcttg tttttttttt taccattatt
     7021 accatcgtgt ttactgttta ttgccccctc aaaaagctaa tgtaattata tttgtgccaa
     7081 taaaaacaag atatgaccta tagaatacaa gtatttcccc ttcgaacatc cccacaagta
     7141 gactttggat ttgtcttcta accaaaagac ttacacacct gcatacctta catcaaaaac
     7201 tcgtttatcg ctacataaaa caccgggata tattttttat atacatactt ttcaaatcgc
     7261 gcgccctctt cataattcac ctccaccaca ccacgtttcg tagttgctct ttcgctgtct
     7321 cccacccgct ctccgcaaca cattcacctt ttgttcgacg accttggagc gactgtcgtt
     7381 agttccgcgc gattcggttc gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa
     7441 attagtaata aatatttgta tgtacaattt atttgctcca atatatttgt atatatttcc
     7501 ctcacagcta tatttattct aatttaatat tatgactttt taaggtaatt ttttgtgacc
     7561 tgttcggagt gattagcgtt acaatttgaa ctgaaagtga catccagtgt ttgttccttg
     7621 tgtagatgca tctcaaaaaa atggtgggca taatagtgtt gtttatatat atcaaaaata
     7681 acaactataa taataagaat acatttaatt tagaaaatgc ttggatttca ctggaactag
     7741 aattaattcg gctgctgctc taaacgacgc atttcgtact ccaaagtacg aattttttcc
     7801 ctcaagctct tattttcatt aaacaatgaa caggacctaa cgcacagtca cgttattgtt
     7861 tacataaatg atttttttta ctattcaaac ttactctgtt tgtgtactcc cactggtata
     7921 gccttctttt atcttttctg gttcaggctc tatcacttta ctaggtacgg catctgcgtt
     7981 gagtcgcctc cttttaaatg tctgaccttt tgcaggtgca gccttccact gcgaatcttt
     8041 aaagtgggta tcacaaattt gggagttttc accaaggctg cacccaaggc tctgctccca
     8101 caattttctc ttaatagcac acttcggcac gtgaattaat tttactccag tcacagcttt
     8161 gcagcaaaat ttgcaatatt tcattttttt ttattccacg taagggttaa tgttttcaaa
     8221 aaaaaattcg tccgcacaca acctttcctc tcaacaagca aacgtgcact gaatttaagt
     8281 gtatacttcg gtaagcttcg gctatcgacg ggaccacctt atgttatttc atcatgggcc
     8341 agacccacgt agtccagcgg cagatcggcg gcggagaagt taagcgtctc caggatgacc
     8401 ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc cggctccacg
     8461 tccgcccatt ggttaatcag cagaccctcg ttggcgtaac ggaaccatga gaggtacgac
     8521 aaccatttga ggtatactgg caccgagccc gagttcaaga agaaggcgtt tttccatagg
     8581 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8641 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8701 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8761 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8821 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8881 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8941 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9001 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9061 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9121 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9181 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9241 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9301 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9361 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9421 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9481 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9541 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9601 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9661 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9721 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9781 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9841 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9901 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9961 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10021 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10081 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10141 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10201 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10261 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10321 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
    10381 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
    10441 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
    10501 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
    10561 ctgagagtgc accatatggg gtgccagggc gtgcccttgg gctccccggg cgcgtatatg
    10621 cggtgtgaaa taccgcaccg aatcgcgcgg aactaacgac agtcgctcca aggtcgtcga
    10681 acaaaaggtg aatgtgttgc ggagagcggg tgggagacag cgaaagagca actacgaaac
    10741 gtggtgtggt ggaggtgaat tatgaagagg gcgcgcgatt tgaaaagtat gtatataaaa
    10801 aatatatccc ggtgttttat gtagcgataa acgagttttt gatgtaaggt atgcaggtgt
    10861 gtaagtcttt tggttagaag acaaatccaa agtctacttg tggggatgtt cgaaggggaa
    10921 atacttgtat tctataggtc atatcttgtt tttattggca caaatataat tacattagct
    10981 ttttgagggg gcaataaaca gtaaacacga tggtaataat ggtaaaaaaa aaaacaagca
    11041 gttatttcgg atatatgtcg gctactcctt gcgtcgggcc cgaagtctta gagccagata
    11101 tgcgagcacc cggaagctca cgatgagaat ggccagacca tgatgaaata acataaggtg
    11161 gtcccgtcgg caagagacat ccacttaacg tatgcttgca ataagtgcga gtgaaaggaa
    11221 tagtattctg agtgtcgtat tgagtctgag tgagacagcg atatgattgt tgattaaccc
    11281 ttagcatgtc cgtggggttt gaattaactc ataatattaa ttagacgaaa ttatttttaa
    11341 agttttattt ttaataattt gcgagtacgc a
//
