LOCUS       sequence-139716-        1076 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian 
            cells. (MBIC5, dL5**, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Telmer CA, Verma R, Teng H, Andreko S, Law L, Bruchez MP
  TITLE     Rapid, specific, no-wash, far-red fluorogen activation in 
            subcellular compartments by targeted fluorogen activating proteins.
  JOURNAL   ACS Chem Biol. 2015 May 15;10(5):1239-46. doi: 10.1021/cb500957k. 
            Epub 2015 Feb 16.
  PUBMED    25650487
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500957k";
            journalName: "ACS Chem Biol"; date: "2015-05-15- 15"; volume: "10"; 
            issue: "5"; pages: "1239-46"
COMMENT     Sequence Label: pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(33..746,747..749,750..752))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     427..446
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acgggccctc tagagtcgac agctgcctcg agttacttgt acagctcgtc catgccgaga
       61 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      121 tctttgctca gcttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
      181 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
      241 ttgcagttga ggccgaagtt ggccttgatg ccgttcttct gcttgtcggc ggtgatatag
      301 acgttgccgt ggatggcgtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      361 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      421 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
      481 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag
      541 cactgcacgc cccagctcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
      601 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
      661 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
      721 ccggtgaaca gctcctcgcc cttgctcacc ataccggtgc tgccaccgcc accagaccca
      781 ccgcctccca tatgggccag accggccgcg gagaggacgg tcagctgggt gcctcctccg
      841 aacagataac cgtcaacgtc ggagagcgaa cagtaatact cagcctcatc ttcaggctgc
      901 gcatccgaga tggtcagggc agccttggcc ccaaggaggg agcctgagaa tcggccaggg
      961 gtccaggaat acttcttgtc ggtgtcaaat ataagtgccc tgggggcttg gccaggcttc
     1021 tgctggaacc agttggcata atgaccactg gtgacagctc cagtgccgga gccaca
//
