LOCUS       sequence-139729-        1069 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic 
            reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, 
            FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Telmer CA, Verma R, Teng H, Andreko S, Law L, Bruchez MP
  TITLE     Rapid, specific, no-wash, far-red fluorogen activation in 
            subcellular compartments by targeted fluorogen activating proteins.
  JOURNAL   ACS Chem Biol. 2015 May 15;10(5):1239-46. doi: 10.1021/cb500957k. 
            Epub 2015 Feb 16.
  PUBMED    25650487
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500957k";
            journalName: "ACS Chem Biol"; date: "2015-05-15- 15"; volume: "10"; 
            issue: "5"; pages: "1239-46"
COMMENT     Sequence Label: pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     145..207
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             214..243
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             259..978
                     /codon_start=1
                     /product="fluorogen-activating protein composed of a dimer 
                     of the L5-MG single-chain antibody fragment (Szent-Gyorgyi 
                     et al., 2008)"
                     /label=FAP-alpha-2
                     /note="binds malachite green derivatives to produce red 
                     fluorescence"
                     /translation="QAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGHYANWFQQKPGQA
                     PRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSLSDVDGYLFGGGT
                     QLTVLSGGGGSGGGGSGGGGSGGGGSQAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGH
                     YANWFQQKPGQAPRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSL
                     SDVDGYLFGGGTQLTVLS"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttgccacc
      121 ggatccggct tggggatatc caccatggag acagacacac tcctgctatg ggtactgctg
      181 ctctgggttc caggttccac tggtgacgaa ttcgagcaga agctgatcag cgaggaggac
      241 ctgggccttt ctggcctgca ggccgtcgtt acccaagaac ctagtgttac cgttagccca
      301 ggtggtactg ttatacttac ttgtggaagt ggtacgggtg ccgtcacatc tggtcattat
      361 gcaaattggt ttcaacaaaa accaggacaa gctccaagag ctttgatttt tgatactgat
      421 aagaagtatt cttggacccc aggtagattt tctggatctt tgctgggagc aaaggcagct
      481 ttgacaatat cagatgctca gcctgaggac gaagccgagt attactgttc tcttagcgac
      541 gtggatggct acttgtttgg cggtggaaca caactgacgg ttctgtccgg tggtggcggc
      601 tctggtggcg gtggcagcgg cggtggtggt tccggaggcg gcggttctca ggctgtggtg
      661 actcaggagc cgtcagtgac tgtgtcccca ggagggacag tcattctcac ttgtggctcc
      721 ggcactggag ctgtcaccag tggtcattat gccaactggt tccagcagaa gcctggccaa
      781 gcccccaggg cacttatatt tgacaccgac aagaagtatt cctggacccc tggccgattc
      841 tcaggctccc tccttggggc caaggctgcc ctgaccatct cggatgcgca gcctgaagat
      901 gaggctgagt attactgttc gctctccgac gttgacggtt atctgttcgg aggaggcacc
      961 cagctgaccg tcctctccgc ggccggtctg gcccatatgg gaggcggtgg gtctggtggc
     1021 ggtggcagca ccggtatggt gagcaagggc gaggagctgt tcaccgggg
//
