LOCUS       sequence-139792-        9372 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Inducible lentiviral expression of Gata6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ngondo RP, Cirera-Salinas D, Yu J, Wischnewski H, Bodak M, 
            Vandormael-Pournin S, Geiselmann A, Wettstein R, Luitz J, 
            Cohen-Tannoudji M, Ciaudo C
  TITLE     Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation
            of Mouse Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023.
  PUBMED    29396181
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023."
COMMENT     Sequence Label: pCW57.1 _Gata6
FEATURES             Location/Qualifiers
     source          1..9372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     79..103
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1086..1106
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     promoter        1894..2404
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2323..2341
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2414..3010
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2414..2433)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2910..2930
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3011..3064
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3065..3682,3683..3688,3689..3724,3725..3727,3728..3763,
                     3764..3766,3767..3802,3803..3811)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3851..3871)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4269..4280)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4485..4718
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4790..4911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4827..4846)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4881..4900
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4951..5086
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5086..5157
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5158..5226,5227..6018)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5376..5395)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6189..6777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6678..6697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6931..6948
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7065..7086
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7101..7106,7107..7124,7125..7131)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7139..7155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7144..7166
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7163..7179
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7163..7179
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7198..7218
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7200..7218
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7246..7472
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7473..7653
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7700..7825
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8318..8551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8736..8780
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8993..9110
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9164..9372,1..106)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9172..9190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9208..9226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9243..9261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9279..9297
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9315..9333
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9350..9368
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
       61 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcca
      121 ccatggcctt gactgacggc ggctggtgcc tgccgaagcg tttcggggct gctgctgcgg
      181 acgccggcga ctccgggccc tttccagcgc gggagccctc ctcgccgctt tcccccatct
      241 cgtcttcgtc ctcctcctgc tcccggggcg gggatcgcgg tccctgcggc gccagcaact
      301 gcaggacgcc gcagctcgac gccgaggcgg tggcgggacc tccgggccgc tcgctcttgc
      361 tcagccccta cgcctcgcat cccttcgccg ctgcccacgg agccgcggcg cccggggtcg
      421 caggccccgg gagcgccctg tcgacttggg aggacctgtt gctcttcact gacctcgatc
      481 aggccgcgac cgccagcaag ctgttgtggt ccagccgggg cgccaaactg agccccttcg
      541 cggccgagca gccggaggaa atgtaccaga ccctcgccgc cctgtccagc caggggcccg
      601 ccgcttacga cggcgcgccc ggcggcttcg tgcactccgc agcggcggcg gccgctgccg
      661 ccgcggcagc cagctcccct gtctacgtgc ccaccacgcg cgtgggctcc atgctgtccg
      721 gcctgcccta ccttcaaggc gcgggcagcg ggcccagcaa tcacgcgggc ggagcgggtg
      781 cccacccagg ctggtcccag gcctccgccg acagcccccc gtatggcggg ggtggcgcag
      841 ccggcggcgg cgcggccgga cctggaggtg ctggatcggc tacggcccac gcctctgcac
      901 ggtttcccta ctcgcccagc ccgcccatgg ccaacggcgc cgcgcgagac cccgggggct
      961 acgtggctgc gggcggcacg ggcgcaggca gtgtgagtgg aggtggcggc agcctggcgg
     1021 ccatgggtgg ccgggagcac cagtacagct cgctgtccgc agctcggccg ctgaacggaa
     1081 cgtaccacca ccaccatcac catcacccga cctactcgcc ctacatggcc gcaccgctga
     1141 ctcctgcctg gccagcagga cccttcgaaa cgccggtgct ccacagctta cagggccgcg
     1201 cgggagctcc actcccggtg ccacgaggcc ccagcacaga cctgttggag gacctgtcgg
     1261 agagccgcga gtgcgtgaac tgcggctcca tccagacgcc actgtggaga cgagacggca
     1321 ccggtcatta cctgtgcaat gcatgcggtc tctacagcaa gatgaatggc ctcagcaggc
     1381 ccctcatcaa gccacagaag cgcgtgcctt catcacggcg gcttggactg tcctgtgcca
     1441 actgtcacac cacaaccact accttatggc gtagaaatgc tgagggtgag cctgtgtgca
     1501 atgcttgcgg gctctatatg aaactccatg gggtgcctcg accacttgct atgaaaaaag
     1561 aaggaattca aaccaggaaa cgaaaaccta aaaatataaa taagtcaaaa gcttgctccg
     1621 gtaacagcag tggctctgtc cctatgactc ctacttcctc ttcttctaat tcagatgact
     1681 gcaccaaaaa tacttctcct tctacacaag cgaccacctc aggggtaggg gcatcagtga
     1741 tgtctgcagt gggagaaaac gccaaccccg agaacagtga cctcaagtat tcaggtcaag
     1801 acggcctcta cataggtgtc agtctgtcct cccctgccga agtcacatcc tccgtgcgac
     1861 aggattcttg gtgtgctctg gccctggcct gacggggttg gggttgcgcc ttttccaagg
     1921 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
     1981 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
     2041 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
     2101 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
     2161 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
     2221 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
     2281 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     2341 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     2401 ccagcaattc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     2461 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     2521 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     2581 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     2641 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     2701 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     2761 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     2821 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     2881 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     2941 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     3001 gcccggtgcc gaaggtagag gttctctcct cacttgtggt gatgttgaag aaaaccctgg
     3061 tccaatgtct agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg
     3121 tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     3181 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     3241 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     3301 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     3361 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     3421 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     3481 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     3541 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     3601 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     3661 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     3721 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     3781 cgattttgac cttgacatgc tccccgggta actaagtaag gatcgatcca agatatcgta
     3841 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3901 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3961 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4021 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4081 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4141 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     4201 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4261 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4321 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4381 ccccgcctgt ttcgcctcgg cgtccggact agaggtacct ttaagaccaa tgacttacaa
     4441 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4501 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4561 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4621 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4681 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     4741 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     4801 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4861 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4921 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4981 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5041 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctttc aaatatgtat
     5101 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5161 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5221 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5281 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5341 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5401 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5461 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5521 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5581 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5641 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5701 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5761 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5821 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5881 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5941 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6001 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6061 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6121 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6181 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6241 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6301 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6361 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6421 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6481 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6541 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6601 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6661 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6721 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6781 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6841 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6901 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     6961 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7021 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7081 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7141 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     7201 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     7261 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     7321 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     7381 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     7441 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     7501 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7561 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7621 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     7681 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     7741 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     7801 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     7861 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     7921 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     7981 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     8041 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     8101 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     8161 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     8221 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     8281 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     8341 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     8401 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     8461 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     8521 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     8581 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     8641 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     8701 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     8761 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     8821 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     8881 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     8941 ccccgagggg acaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa
     9001 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9061 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9121 acagggacag cagagatcca ctttggccgc gaatcgatat gtcgagttta ctccctatca
     9181 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt
     9241 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     9301 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt
     9361 gatagagaac gt
//
